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A scale-down mimic for mapping the process performance of centrifugation, depth and sterile filtration

Joseph, A; Kenty, B; Mollet, M; Hwang, K; Rose, S; Goldrick, S; Bender, J; ... Titchener-Hooker, N; + view all (2016) A scale-down mimic for mapping the process performance of centrifugation, depth and sterile filtration. Biotechnology and Bioengineering , 113 (9) pp. 1934-1941. 10.1002/bit.25967. Green open access

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Abstract

In the production of biopharmaceuticals disk-stack centrifugation is widely used as a harvest step for the removal of cells and cellular debris. Depth filters followed by sterile filters are often then employed to remove residual solids remaining in the centrate. Process development of centrifugation is usually conducted at pilot-scale so as to mimic the commercial scale equipment but this method requires large quantities of cell culture and significant levels of effort for successful characterization. A scale-down approach based upon the use of a shear device and a bench-top centrifuge has been extended in this work towards a preparative methodology that successfully predicts the performance of the continuous centrifuge and polishing filters. The use of this methodology allows the effects of cell culture conditions and large-scale centrifugal process parameters on subsequent filtration performance to be assessed at an early stage of process development where material availability is limited.

Type: Article
Title: A scale-down mimic for mapping the process performance of centrifugation, depth and sterile filtration
Open access status: An open access version is available from UCL Discovery
DOI: 10.1002/bit.25967
Publisher version: http://dx.doi.org/10.1002/bit.25967
Language: English
Additional information: Copyright © 2016 The Authors. Biotechnology and Bioengineering Published by Wiley Periodicals, Inc. This is an open access article under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Keywords: Capillary shear, Centrifugation, Continuous centrifugation, Depth filter, Disk-stack centrifuge, Filter capacity, Mammalian cell, Primary recovery, Scale-down
UCL classification: UCL
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Biochemical Engineering
URI: https://discovery.ucl.ac.uk/id/eprint/1483221
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