Griffiths, VA;
(2016)
Design and implementation of real-time movement correction of brain motion during 3D acousto-optic lens two photon imaging.
Doctoral thesis , UCL (University College London).
Abstract
Two-photon microscopy is widely used in neuroscience due to its ability to image deep within scattering tissue. High speed random access functional imaging with acousto-optic deflectors provides the highest temporal resolution. Unfortunately, random access point measurements in awake animals is particularly prone to movement artefacts, causing misreporting of calcium transients in the monitored points of interest. This thesis describes a new real time method for motion correction that enables imaging of highly magnified features such as dendrites and spines in awake, moving mice. The new architecture exploits the agility of field-programmable gate arrays (FPGAs) to develop new control and data acquisition systems for a 2-photon acousto-optic lens (AOL) microscope. The new architecture enables fast raster scanning at any axial position, axial scanning and “patch” scanning of discrete regions of interest anywhere within the 3D field of view. The real-time movement correction system is capable of sub-micron image stabilization for brain motion of +/- 9mm at frequencies up to 20Hz. Random access functional imaging was demonstrated on dendrites, spines and axons in awake behaving mice.
| Type: | Thesis (Doctoral) |
|---|---|
| Title: | Design and implementation of real-time movement correction of brain motion during 3D acousto-optic lens two photon imaging |
| Event: | University College London |
| Language: | English |
| Keywords: | 2-photon imaging, motion correction, Acousto-Optic Lens |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Neuro, Physiology and Pharmacology |
| URI: | https://discovery.ucl.ac.uk/id/eprint/1477472 |
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