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Mapping the Aggregation Kinetics of a Therapeutic Antibody Fragment

Chakroun, N; Hilton, D; Ahmad, SS; Platt, GW; Dalby, PA; (2016) Mapping the Aggregation Kinetics of a Therapeutic Antibody Fragment. Molecular Pharmaceutics , 13 (2) pp. 307-319. 10.1021/acs.molpharmaceut.5b00387. Green open access

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Abstract

The analytical characterization of biopharmaceuticals is a fundamental step in the early stages of development and prediction of their behavior in bioprocesses. Protein aggregation in particular is a common issue as it affects all stages of product development. In the present work, we investigate the stability and the aggregation kinetics of A33Fab, a therapeutically relevant humanized antibody fragment at a wide range of pH, ionic strength, and temperature. We show that the propensity of A33Fab to aggregate under thermally accelerated conditions is pH and ionic-strength dependent with a stronger destabilizing effect of ionic strength at low pH. In the absence of added salts, A33Fab molecules appear to be protected from aggregation due to electrostatic colloidal repulsion at low pH. Analysis by transmission electron microscopy identified significantly different aggregate species formed at low and high pH. The correlations between apparent midpoints of thermal transitions (Tm,app values), or unfolded mole fractions, and aggregation rates are reported here to be significant only at the elevated incubation temperature of 65 °C, where aggregation from the unfolded state predominates. At all other conditions, particularly at 4-45 °C, aggregation of A33 Fab was predominantly from a native-like state, and the kinetics obeyed Arrhenius behavior. Despite this, the rank order of aggregation rates observed at 45 °C, 23 and 4 °C still did not correlate well to each other, indicating that forced degradation at elevated temperatures was not a good screen for predicting behavior at low temperature.

Type: Article
Title: Mapping the Aggregation Kinetics of a Therapeutic Antibody Fragment
Open access status: An open access version is available from UCL Discovery
DOI: 10.1021/acs.molpharmaceut.5b00387
Publisher version: http://dx.doi.org/10.1021/acs.molpharmaceut.5b0038...
Language: English
Additional information: This is an open access article published under an ACS AuthorChoice License, which permits copying and redistribution of the article or any adaptations for non-commercial purposes.
Keywords: aggregation kinetics, antibody fragment, protein aggregation, protein formulation
UCL classification: UCL
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Biochemical Engineering
URI: https://discovery.ucl.ac.uk/id/eprint/1476051
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