Ibrahim, IM;
Puthiyaveetil, S;
Khan, C;
Allen, JF;
(2016)
Probing the nucleotide-binding activity of a redox sensor: two-component regulatory control in chloroplasts.
Photosynthesis Research
, 130
(1-3)
pp. 93-101.
10.1007/s11120-016-0229-y.
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Abstract
Two-component signal transduction systems mediate adaptation to environmental changes in bacteria, plants, fungi, and protists. Each two-component system consists of a sensor histidine kinase and a response regu- lator. Chloroplast sensor kinase (CSK) is a modified sensor histidine kinase found in chloroplasts—photosynthetic organelles of plants and algae. CSK regulates the tran- scription of chloroplast genes in response to changes in photosynthetic electron transport. In this study, the full- length and truncated forms of Arabidopsis CSK proteins were overexpressed and purified in order to characterise their kinase and redox sensing activities. Our results show that CSK contains a modified kinase catalytic domain that binds ATP with high affinity and forms a quinone adduct that may confer redox sensing activity.
| Type: | Article |
|---|---|
| Title: | Probing the nucleotide-binding activity of a redox sensor: two-component regulatory control in chloroplasts |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1007/s11120-016-0229-y |
| Publisher version: | http://dx.doi.org/ 10.1007/s11120-016-0229-y |
| Language: | English |
| Additional information: | This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
| Keywords: | Two-component regulatory system, Sensor histidine kinase, Response regulator, Chloroplast sensor kinase, Plastoquinone, Redox regulation, Chloroplast DNA, Transcription, Cyanobacteria, Photosystem stoichiometry |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Genetics, Evolution and Environment |
| URI: | https://discovery.ucl.ac.uk/id/eprint/1474740 |
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