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Microcarriers' suspension and flow dynamics in orbitally shaken bioreactors

Pieralisi, I; Rodriguez, G; Micheletti, M; Paglianti, A; Ducci, A; (2016) Microcarriers' suspension and flow dynamics in orbitally shaken bioreactors. Chemical Engineering Research and Design , 108 pp. 198-209. 10.1016/j.cherd.2015.11.020. Green open access

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Abstract

In the present work an effort is made to determine the suspension speed of microcarriers in an orbitally shaken bioreactor of cylindrical geometry, and to assess the associated two-phase flow by means of Particle Image Velocimetry (PIV). Microcarrier technologies are commonly used in the bioprocess industry to culture adherent-dependent cells in three dimensional flow. Commercial GE Cytodex microcarriers were employed throughout this study to best mimic the flow conditions occurring in a bioreactor under standard operating conditions. Suspension speed measurements were obtained at different solid concentrations, that are typical for cell cultures, and for different combinations of orbital to cylinder diameters’ ratio, do/di (c = 2.5 - 12.5 g/L; do/di = 0.2 - 0.7; N = 0 - 200 RPM). The current two-phase PIV results show that mean flow dynamics occurring in the cylindrical bioreactor are not significantly affected by the presence of the microcarriers, and that their suspension is directly associated to the flow transition reported by Weheliye et al. (2013). The flow scaling law included in their study can be successfully employed to predict the full suspension speed across bioreactors of different scales and working under different operating conditions (i.e. inner diameter of the cylinder, di, orbital diameter, do, and filling volume, Vf ).

Type: Article
Title: Microcarriers' suspension and flow dynamics in orbitally shaken bioreactors
Open access status: An open access version is available from UCL Discovery
DOI: 10.1016/j.cherd.2015.11.020
Publisher version: http://dx.doi.org/10.1016/j.cherd.2015.11.020
Language: English
Additional information: Copyright © Elsevier 2015. This article is published under a Creative Commons Attribution Non-commercial Non-derivative 4.0 International license (CC BY-NC-ND 4.0). This license allows you to share, copy, distribute and transmit the work for personal and non-commercial use providing author and publisher attribution is clearly stated. Further details about CC BY licenses are available at http://creativecommons.org/licenses/by/4.0. Access may be initally restricted by the author.
Keywords: Orbitally shaken bioreactor, microcarriers’ suspension speed, PIV, two-phase flow.
UCL classification: UCL
UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Biochemical Engineering
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Mechanical Engineering
URI: https://discovery.ucl.ac.uk/id/eprint/1472917
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