Lizarraga-Valderrama, LR;
Nigmatullin, R;
Taylor, C;
Haycock, JW;
Claeyssens, F;
Knowles, JC;
Roy, I;
(2015)
Nerve tissue engineering using blends of poly(3-hydroxyalkanoates) for peripheral nerve regeneration.
Engineering in Life Sciences
, 15
(6)
pp. 612-621.
10.1002/elsc.201400151.
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Abstract
The only types of polyhydroxyalkanoates (PHAs) that have been explored for use in nerve regeneration are poly(3-hydroxybutyrate), P(3HB), and poly(3- hydroxybutyrate-co-3-hydroxyhexanoate) (P(3HB-co-3HHx)). However, nerve regeneration induced by these PHAs is inferior to that of autologous nerve grafting. The aim of this work was to study novel PHA blends as resorbable biomaterials for the manufacture of nerve guidance conduits. PHA blend films with varying ratios of poly(3-hydroxyoctanoate)/ P(3HB) (P(3HO)/P(3HB)) were produced using the solvent-casting method. Neat films of P(3HO) and P(3HB), along with 25:75, 50:50, and 75:25 blend films of P(3HO)/P(3HB), were characterized with respect to chemical, material, and biological properties. On surface analysis, the blends exhibited higher values of roughness compared with the neat films. The differential scanning calorimetry characterization of the blends confirmed that P(3HO) and P(3HB) formed immiscible blends. FTIR and XRD analysis of the blends showed a decrease in crystallinity along with an increase of the proportion of P(3HO) . However, an increase in the stiffness of the blends was observed when the proportion of P(3HB) increased. Although all of the blends were biocompatible with NG108-15 neuronal cells, the 25:75 P(3HO)/P(3HB) blend showed significantly better support for growth and differentiation of these cells. The mechanical properties of PHA blends correspond to the reported properties of peripheral nerves. Therefore, they could serve as base material for the manufacture of nerve guidance conduits.
| Type: | Article |
|---|---|
| Title: | Nerve tissue engineering using blends of poly(3-hydroxyalkanoates) for peripheral nerve regeneration |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1002/elsc.201400151 |
| Publisher version: | http://dx.doi.org/10.1002/elsc.201400151 |
| Language: | English |
| Additional information: | Copyright © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. This is the peer reviewed version of the following article: Lizarraga-Valderrama, LR; Nigmatullin, R; Taylor, C; Haycock, JW; Claeyssens, F; Knowles, JC; Roy, I; (2015) Nerve tissue engineering using blends of poly(3-hydroxyalkanoates) for peripheral nerve regeneration. Eng. Life Sci.(In press), which has been published in final form at http://dx.doi.org/10.1002/elsc.201400151. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving. |
| Keywords: | Biocompatibility, In vitro test, Nerve regeneration, Neuronal cells, Polyhydroxyalkanoates |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Eastman Dental Institute UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Eastman Dental Institute > Biomaterials and Tissue Eng |
| URI: | https://discovery.ucl.ac.uk/id/eprint/1469346 |
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