UCL Discovery
UCL home » Library Services » Electronic resources » UCL Discovery

Lambert-Eaton syndrome IgG inhibits transmitter release via P/Q Ca21 channels

Kullmann, D; spillane, J; ermolyuk, Y; Cano-Jaimez, M; Lang, B; Vincent, A; Volynski, KE; (2015) Lambert-Eaton syndrome IgG inhibits transmitter release via P/Q Ca21 channels. Neurology , 84 (6) pp. 575-579. ​10.​1212/​WNL.​0000000000001225. Green open access

[thumbnail of Spillane_Kullmann_Neurology2015.pdf]
Preview
Text
Spillane_Kullmann_Neurology2015.pdf
Available under License : See the attached licence file.

Download (1MB)

Abstract

Objective: To determine whether immunoglobulin G (IgG) from patients with Lambert-Eaton myasthenic syndrome (LEMS) decreases action potential–evoked synaptic vesicle exocytosis, and whether the effect is mediated by P/Q-type voltage-gated calcium channels (VGCCs). Methods: IgG was obtained from 4 patients with LEMS (3 males, 1 female), including 2 patients with lung malignancy. Antibodies against P/Q-type VGCCs were detected in all 4 patients, and against N-type VGCCs in 2. We incubated neuronal cultures with LEMS IgG and determined the size of the total recycling pool of synaptic vesicles and the rate of action potential–evoked exocytosis using fluorescence imaging of the amphiphilic dye SynaptoRed C1. Pooled IgG from healthy volunteers was used as a control. We repeated the experiments on synapses lacking P/Qtype calcium channels from a Cacna1a knockout mouse to determine whether these channels account for the pathogenic effect of LEMS IgG. Results: LEMS IgG had no effect on the total recycling pool size but significantly reduced the rate of action potential–evoked synaptic exocytosis in wild-type neurons when compared with neurons treated with control IgG. In contrast, LEMS IgG h

Type: Article
Title: Lambert-Eaton syndrome IgG inhibits transmitter release via P/Q Ca21 channels
Open access status: An open access version is available from UCL Discovery
DOI: ​10.​1212/​WNL.​0000000000001225
Publisher version: http://dx.doi.org/​10.​1212/​WNL.​0000000000001225
Language: English
Additional information: This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
UCL classification: UCL
UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > UCL Queen Square Institute of Neurology
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > UCL Queen Square Institute of Neurology > Clinical and Experimental Epilepsy
URI: https://discovery.ucl.ac.uk/id/eprint/1460568
Downloads since deposit
72Downloads
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item