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A Dual Color Far-Red to Near-Infrared Firefly Luciferin Analogue Designed for Multi-Parametric Bioluminescence Imaging

Jathoul, AP; Grounds, H; Anderson, J; Pule, MA; (2014) A Dual Color Far-Red to Near-Infrared Firefly Luciferin Analogue Designed for Multi-Parametric Bioluminescence Imaging. Angewandte Chemie , 53 (48) pp. 13059-13063. 10.1002/anie.201405955. Green open access

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Abstract

Red-shifted bioluminescent emitters allow improved in vivo tissue penetration and signal quantification, and have led to the development of beetle luciferin analogues that elicit red-shifted bioluminescence with firefly luciferase. However, unlike natural luciferin, none have been shown to emit different colors with different luciferases. We have synthesized and tested the first dual color, far-red to near infrared (nIR) emitting analogue of beetle luciferin, which akin to natural luciferin exhibits pH dependent fluorescence spectra and emits bioluminescence of different colors with different engineered Fluc enzymes. Our analogue produces different far-red to nIR emission maxima with different Fluc mutants up to .max 706 nm. This is the furthest red-shifted form of bioluminescence reported without the requirement of a resonance energy transfer acceptor and such improvement should allow tissues to be more effectively probed using multiparametric deep-tissue bioluminescence imaging.

Type: Article
Title: A Dual Color Far-Red to Near-Infrared Firefly Luciferin Analogue Designed for Multi-Parametric Bioluminescence Imaging
Open access status: An open access version is available from UCL Discovery
DOI: 10.1002/anie.201405955
Publisher version: http://onlinelibrary.wiley.com/journal/10.1002/%28...
Language: English
Additional information: © 2014 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Cancer Institute
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Cancer Institute > Research Department of Haematology
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences > Dept of Chemistry
URI: https://discovery.ucl.ac.uk/id/eprint/1448843
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