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Antibody specificity in neurological disease.

Chapman, M.D.; (2006) Antibody specificity in neurological disease. Doctoral thesis , University of London. Green open access

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Abstract

The study of antigen-specific intrathecal oligoclonal bands is well established and a number of methods have been used to demonstrate that the relative affinity of the antibody produced in multiple sclerosis is low, and in encephalitis, high. A method colloquially known as Eastern blotting was developed whereby relative affinity of individual clones, rather than total antibody, could be studied and quantified by antigen immunoblotting and investigation of a digitised blot using image-manipulating software. This method was used to show that the pixel density of a band in CSF was significantly greater than the same band in serum in a patient with SSPE, and was thus of intrathecal origin. Eastern blotting was then used on a series of samples from a patient with herpes encephalitis to demonstrate that affinity maturation of the immune response had occurred intrathecally. The method was used qualitatively to investigate a proposal that Acinetobacter sp. infection could be the primary cause of multiple sclerosis: no evidence could be found to support the hypothesis. Another suggested cause of multiple sclerosis, Chlamydophila pneumoniae, was studied using a variety of methods including Western blotting. Again, there was no evidence to support the hypothesis. During the project, an unexpected effect of high-strength thiocyanate was revealed, and limited study of this suggested that thiocyanate had an effect on IgG, possibly related to the age of the sample.

Type: Thesis (Doctoral)
Title: Antibody specificity in neurological disease.
Identifier: PQ ETD:592708
Open access status: An open access version is available from UCL Discovery
Language: English
Additional information: Thesis digitised by Proquest Third party copyright material has been removed from the e-thesis.
UCL classification: UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > UCL Queen Square Institute of Neurology
URI: https://discovery.ucl.ac.uk/id/eprint/1445388
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