Logan, N;
Sherif, A;
Cross, AJ;
Collins, SN;
Traynor, A;
Bozec, L;
Parkin, IP;
(2015)
TiO2 -coated CoCrMo: Improving the osteogenic differentiation and adhesion of mesenchymal stem cells in vitro.
J Biomed Mater Res A
, 103
(3)
1208 - 1217.
10.1002/jbm.a.35264.
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Abstract
The current gold standard material for orthopedic applications is titanium (Ti), however, other materials such as cobalt-chromium-molybdenum (CoCrMo) are often preferred due to their wear resistance and mechanical strength. This study investigates if the bioactivity of CoCrMo can be enhanced by coating the surface with titanium oxide (TiO2 ) by atmospheric pressure chemical vapor deposition (CVD), thereby replicating the surface oxide layer found on Ti. CoCrMo, TiO2 -coated CoCrMo (CCMT) and Ti substrates were used for this study. Cellular f-actin distribution was shown to be noticeably different between cells on CCMT and CoCrMo after 24 h in osteogenic culture, with cells on CCMT exhibiting greater spread with developed protrusions. Osteogenic differentiation was shown to be enhanced on CCMT compared to CoCrMo, with increased calcium ion content per cell (p < 0.05), greater hydroxyapatite nodule formation (p < 0.05) and reduced type I collagen deposition per cell (p < 0.05). The expression of the focal adhesion protein vinculin was shown to be marginally greater on CCMT compared to CoCrMo, whereas AFM results indicated that CCMT required more force to remove a single cell from the substrate surface compared to CoCrMo (p < 0.0001). These data suggest that CVD TiO2 coatings may have the potential to increase the biocompatibility of CoCrMo implantable devices. © 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 103A: 1208-1217, 2015.
| Type: | Article |
|---|---|
| Title: | TiO2 -coated CoCrMo: Improving the osteogenic differentiation and adhesion of mesenchymal stem cells in vitro. |
| Location: | United States |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1002/jbm.a.35264 |
| Publisher version: | http://dx.doi.org/10.1002/jbm.a.35264 |
| Language: | English |
| Additional information: | This article is published under a Creative Commons Attribution 4.0 International license (CC BY 4.0). This license allows you to share, copy, distribute and transmit the work for personal and non-commercial use providing author and publisher attribution is clearly stated. Further details about CC BY licenses are available at http://creativecommons.org/ licenses/by/4.0 |
| Keywords: | AFM, cobalt alloy, mesenchymal stem cells, osteogenesis, titanium oxide |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Eastman Dental Institute UCL > Provost and Vice Provost Offices > UCL BEAMS UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences |
| URI: | https://discovery.ucl.ac.uk/id/eprint/1435980 |
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