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Key differences identified between actinic keratosis and cutaneous squamous cell carcinoma by transcriptome profiling

Lambert, SR; Mladkova, N; Gulati, A; Hamoudi, R; Purdie, K; Cerio, R; Leigh, I; ... Harwood, CA; + view all (2014) Key differences identified between actinic keratosis and cutaneous squamous cell carcinoma by transcriptome profiling. British Journal of Cancer , 110 (2) 520 - 529. 10.1038/bjc.2013.760. Green open access

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Abstract

BACKGROUND: Cutaneous squamous cell carcinoma (cSCC) is one of the most common malignancies in fair-skinned populations worldwide and its incidence is increasing. Despite previous observations of multiple genetic abnormalities in cSCC, the oncogenic process remains elusive. The purpose of this study was to elucidate key molecular events associated with progression from premalignant actinic keratoses (AKs) to invasive cSCC by transcriptome profiling. METHODS: We combined laser capture microdissection with the Affymetrix HGU133 Plus 2.0 microarrays to profile 30 cSCC and 10 AKs. RESULTS: We identified a core set of 196 genes that are differentially expressed between AK and cSCC, and are enriched for processes including epidermal differentiation, cell migration, cell-cycle regulation and metabolism. Gene set enrichment analysis highlighted a key role for the mitogen activated protein kinase (MAPK) pathway in cSCC compared with AK. Furthermore, the histological subtype of the tumour was shown to influence the expression profile. CONCLUSION: These data indicate that the MAPK pathway may be pivotal to the transition from AK to cSCC, thus representing a potential target for cSCC prevention. In addition, transcriptome differences identified between cSCC subtypes have important implications for future development of targeted therapies for this malignancy.

Type: Article
Title: Key differences identified between actinic keratosis and cutaneous squamous cell carcinoma by transcriptome profiling
Location: United Kingdom
Open access status: An open access version is available from UCL Discovery
DOI: 10.1038/bjc.2013.760
Publisher version: http://dx.doi.org/10.1038/bjc.2013.760
Language: English
Additional information: Available under the terms of a Creative Commons Attribution-NonCommercial-Share Alike 3.0 Unported License. http://creativecommons.org/licenses/by-nc-sa/3.0/
Keywords: Cutaneous squamous cell carcinoma, MAPK pathway, Actinic keratosis, Expression microarray profiling, Differentiation status Skin cancer
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Surgery and Interventional Sci
URI: https://discovery.ucl.ac.uk/id/eprint/1430949
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