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Evidence from Raman spectroscopy of a putative link between inherent bone matrix chemistry and degenerative joint disease.

Kerns, JG; Gikas, PD; Buckley, K; Shepperd, A; Birch, HL; McCarthy, I; Miles, J; ... Goodship, AE; + view all (2014) Evidence from Raman spectroscopy of a putative link between inherent bone matrix chemistry and degenerative joint disease. Arthritis Rheumatol , 66 (5) pp. 1237-1246. 10.1002/art.38360. Green open access

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Abstract

Objectives. Osteoarthritis (OA) is a common, debilitating disease resulting in degeneration of cartilage and bone in synovial joints. Subtle changes in the molecular structure of subchondral bone matrix occur and may be associated with cartilage changes. This study aims to explore the hypothesis that abnormal molecular changes observed in the matrix of OA subchondral bone can be identified with Raman spectroscopy. Methods. Ten tibial plateaus from patients undergoing total knee replacement for OA were compared with gender and laterality-matched healthy joints undergoing leg amputation (n=5), and non-OA cadaveric specimens (n=5). The latter were age-matched with the OA specimens. The samples were analysed with Raman spectroscopy, peripheral quantitative computed tomography (pQCT) and chemical analysis, comparing defined load-bearing sites in both medial and lateral compartments. Results and Conclusion. The results support our hypothesis that OA subchondral bone matrix changes can be detected by Raman spectroscopy. Furthermore, there is no spectral difference in matrix chemistry between medial and lateral compartments, within each cohort. However, a significant spectral difference (p <0.001) exists between the non-OA and OA specimens. The collagen chain ratios in the non-OA specimens were normal; however, the OA specimens were significantly elevated. The spectroscopy results are compared to multiple standard techniques and indicate, for the first time, that subchondral bone changes, or inherent differences exist in both medial and lateral (beneath intact cartilage) compartments of OA knees. The development of Raman spectroscopy as a screening tool, based on molecular-specific modifications in bone, would facilitate the identification of clinical disease, including early molecular changes. © 2013 American College of Rheumatology.

Type: Article
Title: Evidence from Raman spectroscopy of a putative link between inherent bone matrix chemistry and degenerative joint disease.
Open access status: An open access version is available from UCL Discovery
DOI: 10.1002/art.38360
Publisher version: http://dx.doi.org/10.1002/art.38360
Additional information: © 2014 The Authors. Arthritis & Rheumatology is published by Wiley Periodicals, Inc. on behalf of the American College of Rheumatology. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution, and reproduction in any medium, provided the original work is properly cited.
Keywords: Raman microspectroscopy, osteoarthritis, subchondral bone
UCL classification: UCL
UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Surgery and Interventional Sci
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Surgery and Interventional Sci > Department of Ortho and MSK Science
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Civil, Environ and Geomatic Eng
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Med Phys and Biomedical Eng
URI: https://discovery.ucl.ac.uk/id/eprint/1424068
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