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Visual consequences of molecular changes in the guanylate cyclase activating protein

Stockman, A; Henning, B; Moore, AT; Webster, AR; Michaelides, M; Ripamonti, C; (2014) Visual consequences of molecular changes in the guanylate cyclase activating protein. Investigative Ophthalmology & Visual Science , 55 pp. 1930-1940. 10.1167/iovs.13-13682. Green open access

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Abstract

Purpose: In this study, we characterize and model changes in visual performance associated with a Tyr99Cys substitution in the guanylate cyclase activating protein (GCAP1) in four family members aged between 39 and 55 years. Guanylate cyclase and its activating protein are molecules in the visual transduction pathway that restore cyclic GMP (cGMP) following its light-activated hydrolysis. The mutation causes an excess of cGMP in the dark and results in progressive photoreceptor loss. Methods: L-cone temporal acuity was measured as a function of target irradiance; and L-cone temporal contrast-sensitivity was measured as a function of temporal frequency. Results: All four GCAP1-mutant family members show sensitivity or acuity losses relative to normal observers. The data for the youngest family member are consistent with an abnormal speeding up of the visual response relative to normals, but those for the older members show a progressive higher-frequency sensitivity loss consistent with a slowing down of their response. Conclusions: The speeding up of the visual response in the youngest observer is consistent with the Tyr99Cys-mutation resulting in the more rapid replacement of cGMP after light exposure, and thus in a reduction of temporal integration and relative improvement in high frequency sensitivity compared to normals. The high-frequency losses in the older observers are consistent with their vision being further limited by the interposition of some sluggish process. This might result from some residual or malfunctioning molecular process limiting transduction within damaged photoreceptors, or from an active or passive postreceptoral reorganization caused by the paucity of functioning photoreceptors.

Type: Article
Title: Visual consequences of molecular changes in the guanylate cyclase activating protein
Open access status: An open access version is available from UCL Discovery
DOI: 10.1167/iovs.13-13682
Publisher version: http://dx.doi.org/10.1167/iovs.13-13682
Language: English
Additional information: Copyright © 2014 by Association for Research in Vision and Ophthalmology
UCL classification: UCL
UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > Institute of Ophthalmology
URI: https://discovery.ucl.ac.uk/id/eprint/1420908
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