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RNA polymerase II CTD phosphopeptides compete with RNA for the interaction with Pcf11

Hollingworth, D; Noble, CG; Taylor, IA; Ramos, A; (2006) RNA polymerase II CTD phosphopeptides compete with RNA for the interaction with Pcf11. RNA , 12 (4) pp. 555-560. 10.1261/rna.2304506. Green open access

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Abstract

In Saccharomyces cerevisiae, the cleavage/polyadenylation factor Pcf11 is an important regulatory factor required for recruiting the polyadenylation machinery to the elongating RNA polymerase II (RNAPII) and is necessary for correct transcriptional termination. The interaction with RNAPII is mediated by a CTD-interacting domain (CID) located in the N-terminal region of Pcf11 that binds in a phospho-dependent manner the heptad repeats in the RNAPII CTD. We have previously investigated this protein-protein interaction. We examine here the interaction of the CID with different RNA sequences and look at the effect of phosphopeptides derived from the CTD heptad repeats on the RNA-protein interaction. Our findings demonstrate that the CID displays weak RNA-binding activity, but with some degree of sequence preference, the RNA-protein and peptide-protein interfaces overlap and the CTD-derived phosphopeptides and RNA compete for the binding site. We propose that competition between the protein-peptide and the protein-RNA interaction is important mechanistically and required for the disengagement of polyadenylation factors from RNAPII.

Type: Article
Title: RNA polymerase II CTD phosphopeptides compete with RNA for the interaction with Pcf11
Location: United States
Open access status: An open access version is available from UCL Discovery
DOI: 10.1261/rna.2304506
Publisher version: https://doi.org//10.1261/rna.2304506.
Language: English
Additional information: This version is the version of record. For information on re-use, please refer to the publisher’s terms and conditions.
Keywords: Base Sequence, Binding, Competitive, Nuclear Magnetic Resonance, Biomolecular, RNA Polymerase II, RNA, Fungal, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Surface Plasmon Resonance, mRNA Cleavage and Polyadenylation Factors
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Structural and Molecular Biology
URI: https://discovery.ucl.ac.uk/id/eprint/1418611
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