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Structure of the acceptor stem of Escherichia coli tRNA(Ala): Role of the G3·U70 base pair in synthetase recognition

Ramos, A; Varani, G; (1997) Structure of the acceptor stem of Escherichia coli tRNA(Ala): Role of the G3·U70 base pair in synthetase recognition. Nucleic Acids Research , 25 (11) 2083 - 2090. 10.1093/nar/25.11.2083. Green open access

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Abstract

The fidelity of translation of the genetic code depends on accurate tRNA aminoacylation by cognate aminoacyl-tRNA synthetases. Thus, each tRNA has specificity not only for codon recognition, but also for amino acid identity; this aminoacylation specificity is referred to as tRNA identity. The primary determinant of the acceptor identity of Escherichia coli tRNA(Ala) is a wobble G3·U70 pair within the acceptor stem. Despite extensive biochemical and genetic data, the mechanism by which the G3·U70 pair marks the acceptor end of tRNA(Ala) for aminoacylation with alanine has not been clarified at the molecular level. The solution structure of a microhelix derived from the tRNA(Ala) acceptor end has been determined at high precision using a very extensive set of experimental constraints (≃ 32 per nt) obtained by heteronuclear multidimensional NMR methods. The tRNA(Ala) acceptor end is overall similar to A-form RNA, but important differences are observed. The G3 U70 wobble pair distorts the conformation of the phosphodiester backbone and presents the functional groups of U70 in an unusual spatial location. The discriminator base A73 has extensive stacking overlap with G1 within the G1·C72 base pair at the end of the double helical stem and the -CCA end is significantly less ordered than the rest of the molecule.

Type: Article
Title: Structure of the acceptor stem of Escherichia coli tRNA(Ala): Role of the G3·U70 base pair in synthetase recognition
Open access status: An open access version is available from UCL Discovery
DOI: 10.1093/nar/25.11.2083
Publisher version: http://dx.doi.org/10.1093/nar/25.11.2083
Language: English
Additional information: © 1997 Oxford University Press
UCL classification: UCL
UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Structural and Molecular Biology
URI: https://discovery.ucl.ac.uk/id/eprint/1418598
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