Diaz-Moreno, I;
Hollingworth, D;
Kelly, G;
Martin, S;
Garcia-Mayoral, M;
Briata, P;
Gherzi, R;
(2010)
Orientation of the central domains of KSRP and its implications for the interaction with the RNA targets.
Nucleic Acids Research
, 38
(15)
pp. 5193-5205.
10.1093/nar/gkq216.
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Abstract
KSRP is a multi-domain RNA-binding protein that recruits the exosome-containing mRNA degradation complex to mRNAs coding for cellular proliferation and inflammatory response factors. The selectivity of this mRNA degradation mechanism relies on KSRP recognition of AU-rich elements in the mRNA 30 UTR, that is mediated by KSRP’s KH domains. Our structural analysis shows that the inter-domain linker orients the two central KH domains of KSRP—and their RNA-binding surfaces—creating a two-domain unit. We also show that this inter-domain arrangement is important to the interaction with KSRP’s RNA targets.
| Type: | Article |
|---|---|
| Title: | Orientation of the central domains of KSRP and its implications for the interaction with the RNA targets |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1093/nar/gkq216 |
| Publisher version: | http://doi.org/10.1093/nar/gkq216 |
| Language: | English |
| Additional information: | The Author(s) 2010. Published by Oxford University Press. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/ by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Structural and Molecular Biology |
| URI: | https://discovery.ucl.ac.uk/id/eprint/1418596 |
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