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miR-132 Enhances Dendritic Morphogenesis, Spine Density, Synaptic Integration, and Survival of Newborn Olfactory Bulb Neurons

Pathania, M; Torres-Reveron, J; Yan, L; Kimura, T; Lin, TV; Gordon, V; Teng, ZQ; ... Bordey, A; + view all (2012) miR-132 Enhances Dendritic Morphogenesis, Spine Density, Synaptic Integration, and Survival of Newborn Olfactory Bulb Neurons. PLoS One , 7 (5) , Article e38174. 10.1371/journal.pone.0038174. Green open access

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Abstract

An array of signals regulating the early stages of postnatal subventricular zone (SVZ) neurogenesis has been identified, but much less is known regarding the molecules controlling late stages. Here, we investigated the function of the activity-dependent and morphogenic microRNA miR-132 on the synaptic integration and survival of olfactory bulb (OB) neurons born in the neonatal SVZ. In situ hybridization revealed that miR-132 expression occurs at the onset of synaptic integration in the OB. Using in vivo electroporation we found that sequestration of miR-132 using a sponge-based strategy led to a reduced dendritic complexity and spine density while overexpression had the opposite effects. These effects were mirrored with respective changes in the frequency of GABAergic and glutamatergic synaptic inputs reflecting altered synaptic integration. In addition, timely directed overexpression of miR-132 at the onset of synaptic integration using an inducible approach led to a significant increase in the survival of newborn neurons. These data suggest that miR-132 forms the basis of a structural plasticity program seen in SVZ-OB postnatal neurogenesis. miR-132 overexpression in transplanted neurons may thus hold promise for enhancing neuronal survival and improving the outcome of transplant therapies.

Type: Article
Title: miR-132 Enhances Dendritic Morphogenesis, Spine Density, Synaptic Integration, and Survival of Newborn Olfactory Bulb Neurons
Location: United States
Open access status: An open access version is available from UCL Discovery
DOI: 10.1371/journal.pone.0038174
Publisher version: http://dx.doi.org/10.1371/journal.pone.0038174
Language: English
Additional information: © 2012 Pathania et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. PMCID: PMC3364964
Keywords: Animals, Animals, Newborn, Cell Survival, Cerebral Ventricles, Cyclic AMP Response Element-Binding Protein, Dendritic Spines, Gene Expression Regulation, Developmental, Gene Knockdown Techniques, Mice, MicroRNAs, Neurogenesis, Neuronal Plasticity, Neurons, Olfactory Bulb, Synapses
UCL classification: UCL
UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
URI: https://discovery.ucl.ac.uk/id/eprint/1417168
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