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On the measurement and scaling of mixing time in orbitally shaken bioreactors

Rodriguez, G; Anderlei, T; Micheletti, M; Yianneskis, M; Ducci, A; (2014) On the measurement and scaling of mixing time in orbitally shaken bioreactors. Biochemical Engineering Journal , 82 pp. 10-21. 10.1016/j.bej.2013.10.021. Green open access

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Abstract

Accurate determination of the mixing time in orbitally shaken bioreactors (OSRs) is essential for the optimization of mixing processes and minimization of concentration gradients that can be deleterious to cell cultures. The Dual Indicator System for Mixing Time (DISMT) was employed to measure mixing times in cylindrical and Erlenmeyer flask bioreactors. Various aspects of importance for the acquisition of accurate data from the measurement methodology are discussed, utilizing also comparisons of DISMT and pH probe results obtained in two stirred reactors. The OSR results are juxtaposed with data previously reported in the literature for both cylindrical reactors and Erlenmeyer flasks. The employment of a critical Froude number shows promise for the establishment of a scaling law for mixing time across the various types and sizes of shaken bioreactors.

Type: Article
Title: On the measurement and scaling of mixing time in orbitally shaken bioreactors
Open access status: An open access version is available from UCL Discovery
DOI: 10.1016/j.bej.2013.10.021
Publisher version: http://doi.org/10.1016/j.bej.2013.10.021
Language: English
Additional information: This work is licensed under the Creative Commons Attribution 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by/3.0/
Keywords: Science & Technology, Life Sciences & Biomedicine, Technology, Biotechnology & Applied Microbiology, Engineering, Chemical, Engineering, BIOTECHNOLOGY & APPLIED MICROBIOLOGY, ENGINEERING, CHEMICAL, Shaken bioreactor, Orbital shaking, Mixing time, Mixing, Dual indicator method, Scaling, MONOCLONAL-ANTIBODY, SHAKING BIOREACTORS, STIRRED REACTORS, CELL-CULTURE, HYDRODYNAMICS, ENHANCEMENT, RATES
UCL classification: UCL
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Biochemical Engineering
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Mechanical Engineering
URI: https://discovery.ucl.ac.uk/id/eprint/1416665
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