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Development of a Multiplexed Microfluidic Platform for the Automated Cultivation of Embryonic Stem Cells.

Reichen, M; Veraitch, FS; Szita, N; (2013) Development of a Multiplexed Microfluidic Platform for the Automated Cultivation of Embryonic Stem Cells. J Lab Autom , 18 (6) pp. 519-529. 10.1177/2211068213499917. Green open access

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Abstract

We present a multiplexed platform for a microfabricated stem cell culture device. The modular platform contains all the components to control stem cell culture conditions in an automated fashion. It does not require an incubator during perfusion culture and can be mounted on the stage of an inverted fluorescence microscope for high-frequency imaging of stem cell cultures. A pressure-driven pump provides control over the medium flow rate and offers switching of the flow rates. Flow rates of the pump are characterized for different pressure settings, and a linear correlation between the applied pressure and the flow rate in the cell culture devices is shown. In addition, the pump operates with two culture medium reservoirs, thus enabling the switching of the culture medium on-the-fly during a cell culture experiment. Also, with our platform, the culture medium reservoirs are cooled to prevent medium degradation during long-term experiments. Media temperature is then adjusted to a higher controlled temperature before entering the microfabricated cell culture device. Furthermore, the temperature is regulated in the microfabricated culture devices themselves. Preliminary culture experiments are demonstrated using mouse embryonic stem cells.

Type: Article
Title: Development of a Multiplexed Microfluidic Platform for the Automated Cultivation of Embryonic Stem Cells.
Open access status: An open access version is available from UCL Discovery
DOI: 10.1177/2211068213499917
Publisher version: http://dx.doi.org/10.1177/2211068213499917
Additional information: © 2013 Society for Laboratory Automation and Screening. This article is distributed under the terms of the Creative Commons Attribution 3.0 License (http://www.creativecommons.org/licenses/by/3.0/) which permits any use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access page (http://www.uk.sagepub.com/aboutus/openaccess.htm).
Keywords: bioprocess microfluidics, microfabricated culture device, multiplexing, stem cell culture
UCL classification: UCL
UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Biochemical Engineering
URI: https://discovery.ucl.ac.uk/id/eprint/1403820
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