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The hybrid experimental simplex algorithm – An alternative method for ‘sweet spot’ identification in early bioprocess development: Case studies in ion exchange chromatography

Konstantinidis, S; Chhatre, S; Velayudhan, A; Heldin, E; Titchener-Hooker, N; (2012) The hybrid experimental simplex algorithm – An alternative method for ‘sweet spot’ identification in early bioprocess development: Case studies in ion exchange chromatography. Analytica Chimica Acta , 743 19 - 32. 10.1016/j.aca.2012.06.039. Green open access

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Abstract

The capacity to locate efficiently a subset of experimental conditions necessary for the identification of an operating envelope is a key objective in many studies. We have shown previously how this can be performed by using the simplex algorithm and this paper now extends the approach by augmenting the established simplex method to form a novel hybrid experimental simplex algorithm (HESA) for identifying ‘sweet spots’ during scouting development studies. The paper describes the new algorithm and illustrates its use in two bioprocessing case studies conducted in a 96-well filter plate format. The first investigates the effect of pH and salt concentration on the binding of green fluorescent protein, isolated from Escherichia coli homogenate, to a weak anion exchange resin and the second examines the impact of salt concentration, pH and initial feed concentration upon the binding capacities of a FAb′, isolated from E. coli lysate, to a strong cation exchange resin. Compared with the established algorithm, HESA was better at delivering valuable information regarding the size, shape and location of operating ‘sweet spots’ that could then be further investigated and optimized with follow up studies. To test how favorably these features of HESA compared with conventional DoE (design of experiments) methods, HESA results were also compared with approaches including response surface modeling experimental designs. The results show that HESA can return ‘sweet spots’ that are equivalently or better defined than those obtained from DoE approaches. At the same time the deployment of HESA to identify bioprocess-relevant operating boundaries was accompanied by comparable experimental costs to those of DoE methods. HESA is therefore a viable and valuable alternative route for identifying ‘sweet spots’ during scouting studies in bioprocess development.

Type: Article
Title: The hybrid experimental simplex algorithm – An alternative method for ‘sweet spot’ identification in early bioprocess development: Case studies in ion exchange chromatography
Open access status: An open access version is available from UCL Discovery
DOI: 10.1016/j.aca.2012.06.039
Publisher version: http://dx.doi.org/10.1016/j.aca.2012.06.039
Language: English
Additional information: This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
UCL classification: UCL
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Biochemical Engineering
URI: https://discovery.ucl.ac.uk/id/eprint/1399072
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