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Water scaffolding in collagen: Implications on protein dynamics as revealed by solid-state NMR.

Aliev, AE; Courtier-Murias, D; (2014) Water scaffolding in collagen: Implications on protein dynamics as revealed by solid-state NMR. Biopolymers , 101 (3) pp. 246-256. 10.1002/bip.22330. Green open access

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Abstract

Solid-state NMR studies of collagen samples of various origin confirm that the amplitude of collagen backbone and sidechain motions increases significantly on increasing the water content. This conclusion is supported by the changes observed in three different NMR observables: (i) the linewidth dependence on the (1) H decoupling frequency; (ii) (13) C CSA changes for the peptide carbonyl groups, and (iii) dephasing rates of (1) H-(13) C dipolar couplings. In particular, a nearly three-fold increase in motional amplitudes of the backbone librations about C-C(α) or N-C(α) bonds was found on increasing the added water content up to 47 wt%D2 O. Based on the frequencies of NMR observables involved, the timescale of the protein motions dependent on the added water content is estimated to be of the order of microseconds. This estimate agrees with that from wideline T2 (1) H NMR measurements. Also, our wideline (1) H NMR measurements revealed that the timescale of the microsecond motions in proteins reduces significantly on increasing the added water content, i.e., an approximately 15-fold increase in protein motional frequencies is observed on increasing the added water content to 45 wt%D2 O. The observed changes in collagen dynamics is attributed to the increase in water translational diffusion on increasing the amount of added water, which leads to more frequent "bound water"/"free water" exchange on the protein surface, accompanied by the breakage and formation of new hydrogen bonds with polar functionalities of protein.

Type: Article
Title: Water scaffolding in collagen: Implications on protein dynamics as revealed by solid-state NMR.
Open access status: An open access version is available from UCL Discovery
DOI: 10.1002/bip.22330
Publisher version: http://dx.doi.org/10.1002/bip.22330
Additional information: Copyright © 2013 The Authors Biopolymers Published by Wiley Periodicals, Inc. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
UCL classification: UCL
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences > Dept of Chemistry
URI: https://discovery.ucl.ac.uk/id/eprint/1398215
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