Watkins, AJ;
Hamoudi, RA;
Zeng, N;
Yan, Q;
Huang, Y;
Liu, H;
Zhang, J;
... Du, MQ; + view all
(2012)
An integrated genomic and expression analysis of 7q deletion in splenic marginal zone lymphoma.
PLoS One
, 7
(9)
, Article e44997. 10.1371/journal.pone.0044997.
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Abstract
Splenic marginal zone lymphoma (SMZL) is an indolent B-cell lymphoproliferative disorder characterised by 7q32 deletion, but the target genes of this deletion remain unknown. In order to elucidate the genetic target of this deletion, we performed an integrative analysis of the genetic, epigenetic, transcriptomic and miRNomic data. High resolution array comparative genomic hybridization of 56 cases of SMZL delineated a minimally deleted region (2.8 Mb) at 7q32, but showed no evidence of any cryptic homozygous deletion or recurrent breakpoint in this region. Integrated transcriptomic analysis confirmed significant under-expression of a number of genes in this region in cases of SMZL with deletion, several of which showed hypermethylation. In addition, a cluster of 8 miRNA in this region showed under-expression in cases with the deletion, and three (miR-182/96/183) were also significantly under-expressed (P<0.05) in SMZL relative to other lymphomas. Genomic sequencing of these miRNA and IRF5, a strong candidate gene, did not show any evidence of somatic mutation in SMZL. These observations provide valuable guidance for further characterisation of 7q deletion.
| Type: | Article |
|---|---|
| Title: | An integrated genomic and expression analysis of 7q deletion in splenic marginal zone lymphoma. |
| Location: | United States |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1371/journal.pone.0044997 |
| Publisher version: | http://dx.doi.org/10.1371/journal.pone.0044997 |
| Language: | English |
| Additional information: | © Watkins et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. AJW was supported by a Clinical Research Training Fellowship as part of the Molecular Pathology of Cancer Programme, funded by Cancer Research UK and a research grant from the Addenbrookes Charitable Trust. The work in MQD’s laboratory is supported by research grants from the Leukaemia & Lymphoma Research, UK, the Kay Kendall Leukaemia Fund UK and the National Institute for Health Research Cambridge Biomedical Research Centre. The work in AD’s lab is supported by Lymphoma SPORE (P50 CA097274) Biospecimens Core. QY was supported by a research grant from the National Natural Science Foundation of China (No.30770903). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Surgery and Interventional Sci |
| URI: | https://discovery.ucl.ac.uk/id/eprint/1382228 |
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