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Contact-free scanning and imaging with the scanning ion conductance microscope

Moss, GWJ; Del Linz, SJ; Willman, EJ; Caldwell, M; Klenerman, D; Fernández, FA; (2014) Contact-free scanning and imaging with the scanning ion conductance microscope. Analytical Chemistry , 86 (5) pp. 2353-2360. 10.1021/ac402748j. Green open access

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Abstract

Scanning ion conductance microscopy (SICM) offers the ability to obtain very high resolution topographical images of living cells. In addition, the probe can be used to measure cell responses such as muscle contraction or to map ion channel activity to specific subcellular locations. For biological imaging one of the great advantages of SICM lies in its ability to perform contact-free scanning; imaging cells or tissues without touching the cell surface. However, it is not yet clear when the requirements of this scan mode are met. We have used finite element modelling (FEM) to examine the conditions for contact free scanning and we have validated our modelling using both experimental data and by comparing our results to predictions in analytically tractable situations. Our findings provide a framework for understanding the conditions for contact-free scanning and also extend previous findings with regard to SICM resolution. Finally, we demonstrate the importance of our findings by imaging cells under a variety of conditions.

Type: Article
Title: Contact-free scanning and imaging with the scanning ion conductance microscope
Open access status: An open access version is available from UCL Discovery
DOI: 10.1021/ac402748j
Publisher version: http://dx.doi.org/10.1021/ac402748j
Language: English
Additional information: This work is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
UCL classification: UCL
UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Neuro, Physiology and Pharmacology
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Computer Science
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Electronic and Electrical Eng
URI: https://discovery.ucl.ac.uk/id/eprint/1368262
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