UCL Discovery
UCL home » Library Services » Electronic resources » UCL Discovery

A quantitative assay to measure the relative dna-binding affinity of pyrrolo[2,1-c][1,4]benzodiazepine (pbd) antitumor antibiotics based on the inhibition of restriction-endonuclease bamhi

PUVVADA, MS; HARTLEY, JA; JENKINS, TC; THURSTON, DE; (1993) A quantitative assay to measure the relative dna-binding affinity of pyrrolo[2,1-c][1,4]benzodiazepine (pbd) antitumor antibiotics based on the inhibition of restriction-endonuclease bamhi. Nucleic Acids Research , 21 (16) 3671 - 3675. 10.1093/nar/21.16.3671. Green open access

[thumbnail of nar00065-0074.pdf]
Preview
PDF
nar00065-0074.pdf

Download (911kB)

Abstract

An assay has been developed (restriction endonuclease digestion assay - RED100) based on inhibition of the restriction endonuclease BamHI that is capable of quantitative evaluation of the relative DNA-binding affinity of pyrrolo[2,1-c][1,4]benzodiazepine (PBD) antitumour antibiotics. This method provides comparable results to those obtained from thermal denaturation and ethidium bromide displacement assays but is much more sensitive, discriminating between molecules of similar structure such as DC-81, iso-DC-81 and neothramycin. The results reveal a trend between relative DNA-binding affinity and in vitro cytotoxicity for the PBDs in two tumour cell lines studied.

Type: Article
Title: A quantitative assay to measure the relative dna-binding affinity of pyrrolo[2,1-c][1,4]benzodiazepine (pbd) antitumor antibiotics based on the inhibition of restriction-endonuclease bamhi
Open access status: An open access version is available from UCL Discovery
DOI: 10.1093/nar/21.16.3671
Publisher version: http://dx.doi.org/10.1093/nar/21.16.3671
Language: English
Additional information: The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and Oxford University Press are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use, please contact journals.permissions@oupjournals.org
Keywords: pyrrolo<1,4>benzodiazepine antitumor antibiotics, sequence specificity
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Cancer Institute
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Cancer Institute > Research Department of Oncology
URI: https://discovery.ucl.ac.uk/id/eprint/135973
Downloads since deposit
74Downloads
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item