PUVVADA, MS;
HARTLEY, JA;
JENKINS, TC;
THURSTON, DE;
(1993)
A quantitative assay to measure the relative dna-binding affinity of pyrrolo[2,1-c][1,4]benzodiazepine (pbd) antitumor antibiotics based on the inhibition of restriction-endonuclease bamhi.
Nucleic Acids Research
, 21
(16)
3671 - 3675.
10.1093/nar/21.16.3671.
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Abstract
An assay has been developed (restriction endonuclease digestion assay - RED100) based on inhibition of the restriction endonuclease BamHI that is capable of quantitative evaluation of the relative DNA-binding affinity of pyrrolo[2,1-c][1,4]benzodiazepine (PBD) antitumour antibiotics. This method provides comparable results to those obtained from thermal denaturation and ethidium bromide displacement assays but is much more sensitive, discriminating between molecules of similar structure such as DC-81, iso-DC-81 and neothramycin. The results reveal a trend between relative DNA-binding affinity and in vitro cytotoxicity for the PBDs in two tumour cell lines studied.
| Type: | Article |
|---|---|
| Title: | A quantitative assay to measure the relative dna-binding affinity of pyrrolo[2,1-c][1,4]benzodiazepine (pbd) antitumor antibiotics based on the inhibition of restriction-endonuclease bamhi |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1093/nar/21.16.3671 |
| Publisher version: | http://dx.doi.org/10.1093/nar/21.16.3671 |
| Language: | English |
| Additional information: | The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and Oxford University Press are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use, please contact journals.permissions@oupjournals.org |
| Keywords: | pyrrolo<1,4>benzodiazepine antitumor antibiotics, sequence specificity |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Cancer Institute UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Cancer Institute > Research Department of Oncology |
| URI: | https://discovery.ucl.ac.uk/id/eprint/135973 |
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