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Isolation and characterization of pharmaceutical grade human pentraxins, serum amyloid P component and C-reactive protein, for clinical use.

Pepys, MB; Gallimore, JR; Lloyd, J; Li, Z; Graham, D; Taylor, GW; Ellmerich, S; ... Hawkins, PN; + view all (2012) Isolation and characterization of pharmaceutical grade human pentraxins, serum amyloid P component and C-reactive protein, for clinical use. Journal of Immunological Methods , 384 (1-2) 92 - 102. 10.1016/j.jim.2012.07.013. Green open access

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Abstract

The human pentraxin proteins, serum amyloid P component (SAP) and C-reactive protein (CRP) are important in routine clinical diagnosis, SAP for systemic amyloidosis and CRP for monitoring the non-specific acute phase response. They are also targets for novel therapies currently in development but their roles in health and disease are controversial. Thus, both for clinical use and to rigorously elucidate their functions, structurally and functionally intact, pharmaceutical grade preparations of the natural, authentic proteins are required. We report here the production from normal human donor plasma and the characterization of the first such preparations. Importantly, we demonstrate that, contrary to reports using recombinant proteins and less well characterized preparations, neither CRP nor SAP stimulate the release by human peripheral blood mononuclear cells in vitro of any TNFα, IL-6 or IL-8, nor does SAP cause release of IL-1β or IL-10. Furthermore neither of our preparations was pro-inflammatory in mice in vivo.

Type: Article
Title: Isolation and characterization of pharmaceutical grade human pentraxins, serum amyloid P component and C-reactive protein, for clinical use.
Location: Netherlands
Open access status: An open access version is available from UCL Discovery
DOI: 10.1016/j.jim.2012.07.013
Publisher version: http://dx.doi.org/10.1016/j.jim.2012.07.013
Language: English
Additional information: This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Keywords: Acute-Phase Proteins, Amyloidosis, Animals, C-Reactive Protein, Cells, Cultured, Chromatography, Gel, Cytokines, Electrophoresis, Polyacrylamide Gel, Enzyme-Linked Immunosorbent Assay, Female, Humans, Interleukin-10, Interleukin-1beta, Interleukin-6, Interleukin-8, Leukocytes, Mononuclear, Mice, Mice, Inbred C57BL, Serum Amyloid P-Component, Spectrometry, Mass, Electrospray Ionization, Tumor Necrosis Factor-alpha
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine > Inflammation
URI: https://discovery.ucl.ac.uk/id/eprint/1356470
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