Neal, A;
Boldrin, L;
Morgan, JE;
(2012)
The satellite cell in male and female, developing and adult mouse muscle: distinct stem cells for growth and regeneration.
PLoS One
, 7
(5)
, Article e37950. 10.1371/journal.pone.0037950.
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Abstract
Satellite cells are myogenic cells found between the basal lamina and the sarcolemma of the muscle fibre. Satellite cells are the source of new myofibres; as such, satellite cell transplantation holds promise as a treatment for muscular dystrophies. We have investigated age and sex differences between mouse satellite cells in vitro and assessed the importance of these factors as mediators of donor cell engraftment in an in vivo model of satellite cell transplantation. We found that satellite cell numbers are increased in growing compared to adult and in male compared to female adult mice. We saw no difference in the expression of the myogenic regulatory factors between male and female mice, but distinct profiles were observed according to developmental stage. We show that, in contrast to adult mice, the majority of satellite cells from two week old mice are proliferating to facilitate myofibre growth; however a small proportion of these cells are quiescent and not contributing to this growth programme. Despite observed changes in satellite cell populations, there is no difference in engraftment efficiency either between satellite cells derived from adult or pre-weaned donor mice, male or female donor cells, or between male and female host muscle environments. We suggest there exist two distinct satellite cell populations: one for muscle growth and maintenance and one for muscle regeneration.
| Type: | Article |
|---|---|
| Title: | The satellite cell in male and female, developing and adult mouse muscle: distinct stem cells for growth and regeneration. |
| Location: | United States |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1371/journal.pone.0037950 |
| Publisher version: | http://dx.doi.org/10.1371/journal.pone.0037950 |
| Language: | English |
| Additional information: | © 2012 Neal et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. PMCID: PMC3360677 Jennifer E. Morgan was funded by a Wellcome Trust University Award. Luisa Boldrin was funded by the Muscular Dystrophy Campaign. Alice Neal was funded by the Medical Research Council. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. |
| Keywords: | Age Factors, Animals, Cell Proliferation, Female, Gene Expression Profiling, Male, Mice, Mice, Inbred C57BL, Mice, Inbred mdx, Mice, Nude, Muscle Fibers, Skeletal, Muscle, Skeletal, Myogenic Regulatory Factors, Regeneration, Satellite Cells, Skeletal Muscle, Sex Factors |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL GOS Institute of Child Health UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL GOS Institute of Child Health > Developmental Neurosciences Dept |
| URI: | https://discovery.ucl.ac.uk/id/eprint/1355618 |
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