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Identification of a major rif transcript common to gametocytes and sporozoites of Plasmodium falciparum

Wang, CW; Mwakalinga, SB; Sutherland, CJ; Schwank, S; Sharp, S; Hermsen, CC; Sauerwein, RW; ... Lavstsen, T; + view all (2010) Identification of a major rif transcript common to gametocytes and sporozoites of Plasmodium falciparum. Malaria Journal , 9 , Article 147. 10.1186/1475-2875-9-147. Green open access

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Abstract

Background: The Plasmodium falciparum parasite is transmitted in its sexual gametocyte stage from man to mosquito and as asexual sporozoites from mosquito to man. Developing gametocytes sequester preferentially in the bone marrow, but mature stage gametocytes are released to the bloodstream. Sexual stage parasite surface proteins are of interest as candidate target antigens for transmission blocking vaccines.Methods: In this study, the transcript profiles of rif and var genes, known to encode surface antigens in asexual blood stage parasites, were investigated at different stages of 3D7/NF54 gametocytogenesis and in sporozoites.Results: Gametocytes exhibited a rif transcript profile unlinked to the rif and var transcript profile of the asexual progenitors. At stage V, mature gametocytes produced high levels of a single rif gene, PF13_0006, which also dominated the rif transcript profile of sporozoites. All var genes appeared to be silenced in sporozoites.Conclusions: The most prominent variant surface antigen transcribed in both gametocytes and sporozoites of 3D7/NF54 is a single variant of the RIFIN protein family. This discovery may lead to the identification of the parasites binding ligands responsible for the adhesion during sexual stages and potentially to novel vaccine candidates.

Type: Article
Title: Identification of a major rif transcript common to gametocytes and sporozoites of Plasmodium falciparum
Open access status: An open access version is available from UCL Discovery
DOI: 10.1186/1475-2875-9-147
Publisher version: http://dx.doi.org/10.1186/1475-2875-9-147
Language: English
Additional information: This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Keywords: RED-CELL SURFACE, VAR GENE FAMILY, EXPRESSION PATTERNS, TRANSMISSION STAGES, MALARIA, SEQUESTRATION, ANTIGENS, ERYTHROCYTES, LOCALIZATION, PROTEINS
UCL classification: UCL
UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
URI: https://discovery.ucl.ac.uk/id/eprint/1309947
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