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Implications of storing urinary DNA from different populations for molecular analyses.

Cannas, A; Kalunga, G; Green, C; Calvo, L; Katemangwe, P; Reither, K; Perkins, MD; ... TB trDNA consortium, ; + view all (2009) Implications of storing urinary DNA from different populations for molecular analyses. PLoS One , 4 (9) , Article e6985. 10.1371/journal.pone.0006985. Green open access

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Abstract

Molecular diagnosis using urine is established for many sexually transmitted diseases and is increasingly used to diagnose tumours and other infectious diseases. Storage of urine prior to analysis, whether due to home collection or bio-banking, is increasingly advocated yet no best practice has emerged. Here, we examined the stability of DNA in stored urine in two populations over 28 days.

Type: Article
Title: Implications of storing urinary DNA from different populations for molecular analyses.
Location: United States
Open access status: An open access version is available from UCL Discovery
DOI: 10.1371/journal.pone.0006985
Publisher version: http://dx.doi.org/10.1371/journal.pone.0006985
Language: English
Additional information: © 2009 Cannas et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. The study was funded as part of the TB trDNA European Union framework 6 project 037785. The funders had no role in study design, data collection an analysis, decision to publish or preparation of the manuscript.
Keywords: Adolescent, Adult, Child, Clinical Laboratory Techniques, DNA, Female, Humans, Male, Middle Aged, Reverse Transcriptase Polymerase Chain Reaction, Specimen Handling, Temperature, Time Factors
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Infection and Immunity
URI: https://discovery.ucl.ac.uk/id/eprint/1303230
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