Ryan, CP; Smith, MEB; Schumacher, FF; Grohmann, D; Papaioannou, D; Waksman, G; Werner, F; ... Caddick, S; + view all Ryan, CP; Smith, MEB; Schumacher, FF; Grohmann, D; Papaioannou, D; Waksman, G; Werner, F; Baker, JR; Caddick, S; - view fewer (2011) Tunable reagents for multi-functional bioconjugation: reversible or permanent chemical modification of proteins and peptides by control of maleimide hydrolysis. Chemical Communications , 47 (19) 5452 - 5454. 10.1039/c1cc11114k.

Preview

PDF Ryan_Chemical_Communications_11114k.pdf Download (1MB)

Abstract

Controlling maleimide hydrolysis allows the modular construction of bromomaleimide-mediated bioconjugates which are either stable or cleavable in an aqueous, thiol-mediated reducing environment. The application of this methodology to reversible protein biotinylation, the irreversible labeling of peptide disulfide bonds and the assembly of stable, fluorescein-labelled glycoprotein mimics is described.

Download activity - last month

Export as CSVJSONXML

Download activity - last 12 months

Export as JSONCSVXML

Downloads by country - last 12 months

Export as CSVJSONXML

Archive Staff Only

View Item