UCL Discovery
UCL home » Library Services » Electronic resources » UCL Discovery

GABA_{C} Receptor Sensitivity Is Modulated by Interaction with MAP1B

Billups, D; Hanley, JG; Orme, M; Attwell, D; Moss, SJ; (2000) GABA_{C} Receptor Sensitivity Is Modulated by Interaction with MAP1B. Journal of Neuroscience , 20 (23) 8643 - 8650. 10.1523/JNEUROSCI.20-23-08643.2000. Green open access

[thumbnail of Attwell_GABA_{C} Receptor Sensitivity Is Modulated by Interaction with MAP1B_VoR.pdf]
Preview
Text
Attwell_GABA_{C} Receptor Sensitivity Is Modulated by Interaction with MAP1B_VoR.pdf - Published version

Download (242kB) | Preview

Abstract

GABA_{C} receptors contain ρ subunits and mediate feedback inhibition from retinal amacrine cells to bipolar cells. We previously identified the cytoskeletal protein MAP1B as a ρ1 subunit anchoring protein. Here, we analyze the structural basis and functional significance of the MAP1B-ρ1 interaction. Twelve amino acids at the C terminus of the large intracellular loop of ρ1 (and also ρ2) are sufficient for interaction with MAP1B. Disruption of the MAP1B-ρ interaction in bipolar cells in retinal slices decreased the EC₅₀ of their GABA_{C} receptors, doubling the receptors' current at low GABA concentrations without affecting their maximum current at high concentrations. Thus, anchoring to the cytoskeleton lowers the sensitivity of GABA_{C} receptors and provides a likely site for functional modulation of GABA_{C}receptor-mediated inhibition.

Type: Article
Title: GABA_{C} Receptor Sensitivity Is Modulated by Interaction with MAP1B
Open access status: An open access version is available from UCL Discovery
DOI: 10.1523/JNEUROSCI.20-23-08643.2000
Publisher version: https://doi.org/10.1523/JNEUROSCI.20-23-08643.2000
Language: English
Additional information: This version is the version of record. For information on re-use, please refer to the publisher’s terms and conditions.
Keywords: GABA, ρ, subunit, MAP18, EC₅₀, retina, bipolar, uptake
UCL classification: UCL
UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Neuro, Physiology and Pharmacology
URI: https://discovery.ucl.ac.uk/id/eprint/122692
Downloads since deposit
94Downloads
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item