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Haematopoietic development and immunological function in the absence of cathepsin D

Tulone, C; Uchiyama, Y; Novelli, M; Grosvenor, N; Saftig, P; Chain, BM; (2007) Haematopoietic development and immunological function in the absence of cathepsin D. BMC Immunology , 8 , Article 22. 10.1186/1471-2172-8-22. Green open access

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Abstract

Background: Cathepsin D is a well-characterized aspartic protease expressed ubiquitously in lysosomes. Cathepsin D deficiency is associated with a spectrum of pathologies leading ultimately to death. Cathepsin D is expressed at high levels in many cells of the immune system, but its role in immune function is not well understood. This study examines the reconstitution and function of the immune system in the absence of cathepsin D, using bone marrow radiation chimaeras in which all haematopoietic cells are derived from cathepsin D deficient mice.Results: Cathepsin D deficient bone marrow cells fully reconstitute the major cellular components of both the adaptive and innate immune systems. Spleen cells from cathepsin D deficient chimaeric mice contained an increased number of autofluorescent granules characteristic of lipofuscin positive lysosomal storage diseases. Biochemical and ultrastructural changes in cathepsin D deficient spleen are consistent with increased autolysosomal activity. Chimaeric mice were immunised with either soluble (dinitrophenylated bovine gamma globulin) or particulate (sheep red blood cells) antigens. Both antigens induced equivalent immune responses in wild type or cathepsin D deficient chimaeras.Conclusion: All the parameters of haematopoietic reconstitution and adaptive immunity which were measured in this study were found to be normal in the absence of cathepsin D, even though cathepsin D deficiency leads to dysregulation of lysosomal function.

Type: Article
Title: Haematopoietic development and immunological function in the absence of cathepsin D
Open access status: An open access version is available from UCL Discovery
DOI: 10.1186/1471-2172-8-22
Publisher version: http://dx.doi.org/10.1186/1471-2172-8-22
Language: English
Additional information: © 2007 Tulone et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Keywords: NEURONAL CEROID-LIPOFUSCINOSES, D DEFICIENCY, ANTIGEN PRESENTATION, LYSOSOMAL STORAGE, DENDRITIC CELLS, APOPTOSIS, NEURODEGENERATION, MACROPHAGES, INVOLVEMENT, ACTIVATION
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Infection and Immunity
URI: https://discovery.ucl.ac.uk/id/eprint/111788
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