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Generation of immunocompetent somatic glioblastoma mouse models through in situ transformation of subventricular zone neural stem cells

Clements, Melanie; Simpson Ragdale, Holly; Garcia-Diaz, Claudia; Parrinello, Simona; (2024) Generation of immunocompetent somatic glioblastoma mouse models through in situ transformation of subventricular zone neural stem cells. STAR Protocols , 5 (1) , Article 102928. 10.1016/j.xpro.2024.102928. Green open access

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Abstract

Disease-relevant in vivo tumor models are essential tools for both discovery and translational research. Here, we describe a highly genetically tractable technique for generating immunocompetent somatic glioblastoma (GBM) mouse models using piggyBac transposition and CRISPR-Cas9-mediated gene editing in wild-type mice. We describe steps to deliver plasmids into subventricular zone endogenous neural stem cells by injection and electroporation, leading to the development of adult tumors that closely recapitulate the histopathological, molecular, and cellular features of human GBM. For complete details on the use and execution of this protocol, please refer to Garcia-Diaz et al.1.

Type: Article
Title: Generation of immunocompetent somatic glioblastoma mouse models through in situ transformation of subventricular zone neural stem cells
Location: United States
Open access status: An open access version is available from UCL Discovery
DOI: 10.1016/j.xpro.2024.102928
Publisher version: http://dx.doi.org/10.1016/j.xpro.2024.102928
Language: English
Additional information: © 2024 The Authors. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
Keywords: CRISPR, Cancer, Model Organisms, Stem Cells
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Cancer Institute
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Cancer Institute > Research Department of Cancer Bio
URI: https://discovery.ucl.ac.uk/id/eprint/10188966
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