Ghosh, Pria N;
Verster, Ruhan;
Sewell, Thomas R;
O'Hanlon, Simon J;
Brookes, Lola M;
Rieux, Adrien;
Garner, Trenton WJ;
... Fisher, Matthew C; + view all
(2021)
Discriminating lineages of Batrachochytrium dendrobatidis using quantitative PCR.
Molecular Ecology Resources
, 21
(5)
pp. 1452-1459.
10.1111/1755-0998.13299.
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Abstract
The ability to detect and monitor infectious disease in a phylogenetically informative manner is critical for their management. Phylogenetically informative diagnostic tests enable patterns of pathogen introduction or changes in the distribution of genotypes to be measured, enabling research into the ecology of the pathogen. Batrachochytrium dendrobatidis (Bd), a causative agent of chytridiomycosis in amphibian populations, emerged worldwide in the 21st century and is composed of six lineages which are display varying levels of virulence in their hosts. Research into the distribution, ecology and pathogenicity of these lineages has been hampered by an inability to type lineage efficiently. Here, we describe a lineage-specific TaqMan qPCR assay that differentiates the two lineages of Bd most commonly associated with chytridiomycosis: BdGPL and BdCAPE. We demonstrate how this assay can be used for the surveillance of wild populations of amphibians in Southern Africa using skin swabs, tissue samples and cultured isolates.
| Type: | Article |
|---|---|
| Title: | Discriminating lineages of Batrachochytrium dendrobatidis using quantitative PCR |
| Location: | England |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1111/1755-0998.13299 |
| Publisher version: | http://dx.doi.org/10.1111/1755-0998.13299 |
| Language: | English |
| Additional information: | © 2020 The Authors. Molecular Ecology Resources published by John Wiley & Sons Ltd This is an open access article under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/). |
| Keywords: | Batrachochytrium dendrobatidis, chytrid, diagnostic, lineage, qPCR |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Genetics, Evolution and Environment |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10188853 |
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