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In vitro B cell experiments explore the role of CD24, CD38, and energy metabolism in ME/CFS

Armstrong, CW; Mensah, FFK; Leandro, MJ; Reddy, V; Gooley, PR; Berkovitz, S; Cambridge, G; (2024) In vitro B cell experiments explore the role of CD24, CD38, and energy metabolism in ME/CFS. Frontiers in Immunology , 14 , Article 1178882. 10.3389/fimmu.2023.1178882. Green open access

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Abstract

Introduction: Disturbances of energy metabolism contribute to the clinical manifestations of myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS). Previously, we found that B cells from ME/CFS patients have an increased expression of CD24, a modulator of many cellular functions including those of cell stress. The relative ability of B cells from ME/CFS patients and healthy controls (HC) to respond to rapid changes in energy demand was compared. Methods: CD24, the ectonucleotidases CD39 and CD73, the NAD-degrading enzyme CD38, and mitochondrial mass (MM) were measured following cross-linking of the B cell receptor and costimulation with either T-cell-dependent or Toll-like-receptor-9-dependent agonists. The levels of metabolites consumed/produced were measured using 1H-NMR spectroscopy and analyzed in relation to cell growth and immunophenotype. Results: Proliferating B cells from patients with ME/CFS showed a lower mitochondrial mass and a significantly increased usage of essential amino acids compared with those from HC, with a significantly delayed loss of CD24 and an increased expression of CD38 following stimulation. Discussion: The immunophenotype results suggested the triggering of a stress response in ME/CFS B cells associated with the increased usage of additional substrates to maintain necessary ATP levels. Disturbances in energy metabolism in ME/CFS B cells were thus confirmed in a dynamic in vitro model, providing the basis for further mechanistic investigations.

Type: Article
Title: In vitro B cell experiments explore the role of CD24, CD38, and energy metabolism in ME/CFS
Open access status: An open access version is available from UCL Discovery
DOI: 10.3389/fimmu.2023.1178882
Publisher version: http://dx.doi.org/10.3389/fimmu.2023.1178882
Language: English
Additional information: Copyright © 2024 Armstrong, Mensah, Leandro, Reddy, Gooley, Berkovitz and Cambridge. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
Keywords: chronic fatigue syndrome, B cells, metabolomics, CD24, metabolism
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine > Inflammation
URI: https://discovery.ucl.ac.uk/id/eprint/10186427
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