Verma, Damini;
Yadav, Sumit K;
Kalkal, Ashish;
Pradhan, Rangadhar;
Packirisamy, Gopinath;
(2023)
An Ultrasensitive Electrochemical Immunosensor Comprising Green Synthesized α-Fe2O3NPs_rGO Nanocomposite for Determination of Oral Cancer.
IEEE Sensors Letters
, 7
(12)
, Article 4503904. 10.1109/LSENS.2023.3330915.
Preview |
Text
SENL.pdf - Accepted Version Download (1MB) | Preview |
Abstract
Oral squamous cell carcinoma is a commonly known malignant tumor in the maxillofacial and oral region with a poor prognosis. Therefore, in the present letter, we have developed for the first-time screen-printed electrode (SPE)-based affordable, simple, and ultrasensitive electrochemical immunosensor using a green synthesized hematite nanoparticles (α-Fe 2 O 3 NPs) supported on reduced graphene oxide (rGO) nanocomposite for determination of CYFRA-21-1 cancer biomarker. The α-Fe 2 O 3 NPs_rGO composite has been prepared using a green method employing the leaves extract of Cinnamomum tamala . The 3-aminopropyl triethoxysilane (APTES) helped in the functionalization of α-Fe 2 O 3 NPs_rGO nanocomposite and was drop casted to the working area of SPE, followed by immobilization with the anti-CYFRA-21-1 antibodies as well as bovine serum albumin (BSA) to form BSA/anti-CYFRA-21-1/APTES/α-Fe 2 O 3 NPs_rGO/SPE immunoplatform. The α-Fe 2 O 3 NPs_rGO nanocomposite was characterized by utilizing the Fourier transform infrared spectroscopy, X-ray diffraction, differential pulse voltammetry, and cyclic voltammetry for investigating the crystal structure, as well as electrochemical properties. The developed immunosensor depicted remarkable electrochemical properties having broad linearity (0.5–20 ng/mL), limit of quantification of 0.048 ng/mL, low detection limit of 0.014 ng/mL, and high sensitivity of 90.42 µA (log (ng/mL) −1 cm −2 . In addition, it showed high reproducibility and good selectivity towards the CYFRA-21-1 biomarker. Thus, this letter unlocks innovative prospects for exploring the electrochemical behavior of green synthesized α-Fe 2 O 3 NPs_rGO and its efficacy for fabricating electrochemical biosensors, as well as point of care sensing devices.
| Type: | Article |
|---|---|
| Title: | An Ultrasensitive Electrochemical Immunosensor Comprising Green Synthesized α-Fe2O3NPs_rGO Nanocomposite for Determination of Oral Cancer |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1109/LSENS.2023.3330915 |
| Publisher version: | http://dx.doi.org/10.1109/lsens.2023.3330915 |
| Language: | English |
| Additional information: | This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions. |
| Keywords: | Chemical and biological sensors; electrochemical detection; immunosensor; nanocomposite; oral cancer |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > UCL BEAMS UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Mechanical Engineering |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10185510 |
Archive Staff Only
 |
View Item |
