Benedetti, Giada;
Fournon Berodia, Beatriz;
De Coppi, Paolo;
Giobbe, Giovanni;
(2023)
Human gastro-intestinal organoid engineering: a state of the art.
Frontiers in Chemical Engineering
, 5
, Article 1256334. 10.3389/fceng.2023.1256334.
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Abstract
Gastrointestinal organ failure, from congenital or postnatally acquired pathologies, is a major cause of death across countries of all income levels. Organoids and engineered tissues have been widely investigated as tools to model organ functions and treat pathologies. In this review we aim to describe the progress in human organoid engineering applied to the gastrointestinal tract (namely esophagus, stomach, and intestine). Starting from the onset of the organoid culture technique, we illustrate genetic engineering, stem cell niche engineering, bioprinting, and microfluidics approaches used to integrate mechano-physiological parameters with human organoids. Thanks to these improvements, organoid technology allows disease modelling of patient-specific pathologies, and personalized treatment screening, also offering a cell source for autologous transplantation. We further present an overview of the advances of tissue engineering in animal systems, concerning novel materials and scaffolds to be combined with a variety of cell types to reconstitute a viable surrogate for implantation. The effort in this field sets organoids as an important tool in personalized and regenerative medicine. Their application combined with the advances in tissue engineering holds great potential for translational application.
| Type: | Article |
|---|---|
| Title: | Human gastro-intestinal organoid engineering: a state of the art |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.3389/fceng.2023.1256334 |
| Publisher version: | https://doi.org/10.3389/fceng.2023.1256334 |
| Language: | English |
| Additional information: | © 2023 Benedetti, Fournon Berodia, De Coppi and Giobbe. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
| Keywords: | Human organoids, tissue engineering, GI tract, esophagus, stomach, intestine |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL GOS Institute of Child Health UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL GOS Institute of Child Health > Developmental Biology and Cancer Dept |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10180531 |
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