UCL Discovery
UCL home » Library Services » Electronic resources » UCL Discovery

Affordable optical clearing and immunolabelling in mouse brain slices

Muza, PM; Pérez, M; Noy, S; Kurosawa, M; Katsouri, L; Tybulewicz, VLJ; Fisher, EMC; (2023) Affordable optical clearing and immunolabelling in mouse brain slices. BMC research notes , 16 (1) , Article 246. 10.1186/s13104-023-06511-y. Green open access

[thumbnail of Katsouri_Affordable optical clearing and immunolabelling in mouse brain slices_VoR.pdf]
Preview
Text
Katsouri_Affordable optical clearing and immunolabelling in mouse brain slices_VoR.pdf - Published Version

Download (1MB) | Preview

Abstract

Traditional histological analysis is conducted on thin tissue sections, limiting the data capture from large tissue volumes to 2D profiles, and requiring stereological methods for 3D assessment. Recent advances in microscopical and tissue clearing methods have facilitated 3D reconstructions of tissue structure. However, staining of large tissue blocks remains a challenge, often requiring specialised and expensive equipment to clear and immunolabel tissue. Here, we present the Affordable Brain Slice Optical Clearing (ABSOC) method: a modified iDISCO protocol which enables clearing and immunolabeling of mouse brain slices up to 1 mm thick using inexpensive reagents and equipment, with no intensive expert training required. We illustrate the use of ABSOC in 1 mm C57BL/6J mouse coronal brain slices sectioned through the dorsal hippocampus and immunolabelled with an anti-calretinin antibody. The ABSOC method can be readily used for histological studies of mouse brain in order to move from the use of very thin tissue sections to large volumes of tissue - giving more representative analysis of biological samples, without the need for sampling of small regions only.

Type: Article
Title: Affordable optical clearing and immunolabelling in mouse brain slices
Location: England
Open access status: An open access version is available from UCL Discovery
DOI: 10.1186/s13104-023-06511-y
Publisher version: https://doi.org/10.1186/s13104-023-06511-y
Language: English
Additional information: Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
Keywords: ABSOC, Calretinin, Immunolabeling, Optical Clearing, iDISCO, Mice, Animals, Mice, Inbred C57BL, Brain, Microscopy, Imaging, Three-Dimensional, Specimen Handling
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > UCL Queen Square Institute of Neurology
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > The Sainsbury Wellcome Centre
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > UCL Queen Square Institute of Neurology > Department of Neuromuscular Diseases
URI: https://discovery.ucl.ac.uk/id/eprint/10178680
Downloads since deposit
10Downloads
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item