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A genetic screen in Drosophila reveals the role of fucosylation in host susceptibility to Candida infection

Glittenberg, MT; Kounatidis, I; Atilano, M; Ligoxygakis, P; (2022) A genetic screen in Drosophila reveals the role of fucosylation in host susceptibility to Candida infection. DMM Disease Models & Mechanisms , 15 (5) , Article dmm049218. 10.1242/dmm.049218. Green open access

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Abstract

Candida infections constitute a blind spot in global public health as very few new anti-fungal drugs are being developed. Genetic surveys of host susceptibilities to such infections using mammalian models have certain disadvantages in that obtaining results is time-consuming, owing to relatively long lifespans, and these results have low statistical resolution because sample sizes are usually small. Here, we report a targeted genetic screening of 5698 RNAi lines encompassing 4135 Drosophila genes with human homologues, several of which we identify as important for host survival after Candida albicans infection. These include genes in a variety of functional classes encompassing gene expression, intracellular signalling, metabolism and enzymatic regulation. Analysis of one of the screen hits, the infection-induced α-(1,3)fucosylase FucTA, showed that N-glycan fucosylation has several targets among proteins involved in host defence, which provides multiple avenues of investigation for the mechanistic analysis of host survival to systemic C. albicans infection.

Type: Article
Title: A genetic screen in Drosophila reveals the role of fucosylation in host susceptibility to Candida infection
Location: England
Open access status: An open access version is available from UCL Discovery
DOI: 10.1242/dmm.049218
Publisher version: https://doi.org/10.1242/dmm.049218
Language: English
Additional information: © 2022. Published by The Company of Biologists Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0).
Keywords: Candida albicans, Drosophila, Genetic screen, Fucosylation, Host-pathogen interaction
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Genetics, Evolution and Environment
URI: https://discovery.ucl.ac.uk/id/eprint/10177046
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