UCL Discovery
UCL home » Library Services » Electronic resources » UCL Discovery

Discovery and validation of serum glycoprotein biomarkers for high grade serous ovarian cancer

Dutt, Mriga; Hartel, Gunter; Richards, Renee S; Shah, Alok K; Mohamed, Ahmed; Apostolidou, Sophia; Gentry-Maharaj, Aleksandra; ... Hill, Michelle M; + view all (2023) Discovery and validation of serum glycoprotein biomarkers for high grade serous ovarian cancer. Proteomics: Clinical Applications , Article 2200114. 10.1002/prca.202200114. (In press). Green open access

[thumbnail of Discovery and validation of serum glycoprotein biomarkers for high grade serous.pdf]
Preview
PDF
Discovery and validation of serum glycoprotein biomarkers for high grade serous.pdf - Published Version

Download (2MB) | Preview

Abstract

Purpose: This study aimed to identify serum glycoprotein biomarkers for early detection of high-grade serous ovarian cancer (HGSOC), the most common and aggressive histotype of ovarian cancer./ Experimental design: The glycoproteomics pipeline lectin magnetic bead array (LeMBA)-mass spectrometry (MS) was used in age-matched case-control serum samples. Clinical samples collected at diagnosis were divided into discovery (n = 30) and validation (n = 98) sets. We also analysed a set of preclinical sera (n = 30) collected prior to HGSOC diagnosis in the UK Collaborative Trial of Ovarian Cancer Screening./ Results: A 7-lectin LeMBA-MS/MS discovery screen shortlisted 59 candidate proteins and three lectins. Validation analysis using 3-lectin LeMBA-multiple reaction monitoring (MRM) confirmed elevated A1AT, AACT, CO9, HPT and ITIH3 and reduced A2MG, ALS, IBP3 and PON1 glycoforms in HGSOC. The best performing multimarker signature had 87.7% area under the receiver operating curve, 90.7% specificity and 70.4% sensitivity for distinguishing HGSOC from benign and healthy groups. In the preclinical set, CO9, ITIH3 and A2MG glycoforms were altered in samples collected 11.1 ± 5.1 months prior to HGSOC diagnosis, suggesting potential for early detection./ Conclusions and clinical relevance: Our findings provide evidence of candidate early HGSOC serum glycoprotein biomarkers, laying the foundation for further study in larger cohorts.

Type: Article
Title: Discovery and validation of serum glycoprotein biomarkers for high grade serous ovarian cancer
Location: Germany
Open access status: An open access version is available from UCL Discovery
DOI: 10.1002/prca.202200114
Publisher version: https://doi.org/10.1002/prca.202200114
Language: English
Additional information: © 2023 The Authors. Proteomics – Clinical Applications published by Wiley-VCH GmbH This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Keywords: high grade serous ovarian cancer, lectin magnetic bead array (LeMBA), mass spectrometry, ovarian cancer screening, serum glycoprotein biomarker
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > Inst of Clinical Trials and Methodology
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > Inst of Clinical Trials and Methodology > MRC Clinical Trials Unit at UCL
URI: https://discovery.ucl.ac.uk/id/eprint/10171913
Downloads since deposit
20Downloads
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item