Fisher, Kimone L;
Moodley, Denelle;
Rajkumar-Bhugeloo, Kerishka;
Baiyegunhi, Omolara O;
Karim, Farina;
Ndlovu, Hlumani;
Ndung'u, Thumbi;
(2022)
Elevated IP-10 at the Protein and Gene Level Associates With Pulmonary TB.
Frontiers in Cellular and Infection Microbiology
, 12
, Article 908144. 10.3389/fcimb.2022.908144.
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Abstract
There is an urgent need for accurate and sensitive diagnostic tools that can overcome the current challenge to distinguish individuals with latent tuberculosis infection (LTBI) from individuals with active tuberculosis (TB). Recent literature has suggested that a group of cytokines may serve as biomarkers of TB disease progression. Using a multiplex ELISA, we quantified 27 circulatory markers present within the unstimulated plasma of individuals in Durban, South Africa who were healthy (n=20), LTBI (n=13), or had active TB (n=30). RT-qPCR was performed to measure gene expression of the cytokines of interest, using RNA isolated from healthy (n=20), LTBI (n=20), or active TB (n=30). We found that at the protein level, IL-1RA, IL-6, and IP-10 were significantly more abundant in participants with active TB (p< 0.05) compared to those with LTBI individuals. IP-10 also showed the strongest association with active TB compared to healthy and LTBI at mRNA level. Our data shows that these proteins may serve as biomarkers of TB at both the protein and gene level.
| Type: | Article |
|---|---|
| Title: | Elevated IP-10 at the Protein and Gene Level Associates With Pulmonary TB |
| Location: | Switzerland |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.3389/fcimb.2022.908144 |
| Publisher version: | https://doi.org/10.3389/fcimb.2022.908144 |
| Language: | English |
| Additional information: | This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third-party material in this article are included in the Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
| Keywords: | Science & Technology, Life Sciences & Biomedicine, Immunology, Microbiology, latent TB, tuberculosis, biomarkers, IP-10, TB diagnosis, IL-1RA, disease progression, TUMOR-NECROSIS-FACTOR, IMMUNE-RESPONSES, DISEASE-ACTIVITY, IFN-GAMMA, ACTIVE TB, TUBERCULOSIS, BIOMARKERS, DIAGNOSIS, INFECTION, ALPHA |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Infection and Immunity |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10167741 |
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