Pirkalkhoran, S;
Grabowska, WR;
Kashkoli, HH;
Mirhassani, R;
Guiliano, D;
Dolphin, C;
Khalili, H;
(2023)
Bioengineering of Antibody Fragments: Challenges and Opportunities.
Bioengineering
, 10
(2)
, Article 122. 10.3390/bioengineering10020122.
Preview |
Text
bioengineering-10-00122.pdf - Published Version Download (1MB) | Preview |
Abstract
Antibody fragments are used in the clinic as important therapeutic proteins for treatment of indications where better tissue penetration and less immunogenic molecules are needed. Several expression platforms have been employed for the production of these recombinant proteins, from which E. coli and CHO cell-based systems have emerged as the most promising hosts for higher expression. Because antibody fragments such as Fabs and scFvs are smaller than traditional antibody structures and do not require specific patterns of glycosylation decoration for therapeutic efficacy, it is possible to express them in systems with reduced post-translational modification capacity and high expression yield, for example, in plant and insect cell-based systems. In this review, we describe different bioengineering technologies along with their opportunities and difficulties to manufacture antibody fragments with consideration of stability, efficacy and safety for humans. There is still potential for a new production technology with a view of being simple, fast and cost-effective while maintaining the stability and efficacy of biotherapeutic fragments.
Type: | Article |
---|---|
Title: | Bioengineering of Antibody Fragments: Challenges and Opportunities |
Open access status: | An open access version is available from UCL Discovery |
DOI: | 10.3390/bioengineering10020122 |
Publisher version: | https://doi.org/10.3390/bioengineering10020122 |
Language: | English |
Additional information: | © 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
Keywords: | bioengineering; biotherapeutics; antibody fragments; baculovirus expression system; plants expression system |
UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > UCL School of Pharmacy UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > UCL School of Pharmacy > Pharmaceutics |
URI: | https://discovery.ucl.ac.uk/id/eprint/10166694 |
Archive Staff Only
View Item |