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A gene regulatory network for neural induction

Trevers, Katherine E; Lu, Hui-Chun; Yang, Youwen; Thiery, Alexandre P; Strobl, Anna C; Anderson, Claire; Pálinkášová, Božena; ... Stern, Claudio D; + view all (2023) A gene regulatory network for neural induction. eLife , 12 , Article e73189. 10.7554/eLife.73189. (In press). Green open access

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Abstract

During early vertebrate development, signals from a special region of the embryo, the organizer, can re-direct the fate of non-neural ectoderm cells to form a complete, patterned nervous system. This is called neural induction and has generally been imagined as a single signalling event, causing a switch of fate. Here we undertake a comprehensive analysis, in very fine time-course, of the events following exposure of competent ectoderm of the chick to the organizer (the tip of the primitive streak, Hensen's node). Using transcriptomics and epigenomics we generate a Gene Regulatory Network comprising 175 transcriptional regulators and 5,614 predicted interactions between them, with fine temporal dynamics from initial exposure to the signals to expression of mature neural plate markers. Using in situ hybridization, single-cell RNA-sequencing and reporter assays we show that the gene regulatory hierarchy of responses to a grafted organizer closely resembles the events of normal neural plate development. The study is accompanied by an extensive resource, including information about conservation of the predicted enhancers in other vertebrates.

Type: Article
Title: A gene regulatory network for neural induction
Location: England
Open access status: An open access version is available from UCL Discovery
DOI: 10.7554/eLife.73189
Publisher version: https://doi.org/10.7554/eLife.73189
Language: English
Additional information: Copyright © 2023, Trevers et al. This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
Keywords: chicken, developmental biology
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Cell and Developmental Biology
URI: https://discovery.ucl.ac.uk/id/eprint/10166123
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