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GliaMorph: A modular image analysis toolkit to quantify Müller glial cell morphology

Kugler, Elisabeth; Bravo, Isabel; Durmishi, Xhuljana; Marcotti, Stefania; Beqiri, Sara; Carrington, Alicia; Stramer, Brian M; ... MacDonald, Ryan B; + view all (2023) GliaMorph: A modular image analysis toolkit to quantify Müller glial cell morphology. Development , 150 (3) , Article dev.201008. 10.1242/dev.201008. Green open access

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Abstract

Cell morphology is critical for all cell functions. This is particularly true for glial cells as they rely on complex shape to contact and support neurons. However, methods to quantify complex glial cell shape accurately and reproducibly are lacking. To address this, we developed the image analysis pipeline "GliaMorph". GliaMorph is a modular analysis toolkit developed to perform (i) image pre-processing, (ii) semi-automatic region-of-interest (ROI) selection, (iii) apicobasal texture analysis, (iv) glia segmentation, and (v) cell feature quantification. Müller Glia (MG) have a stereotypic shape linked to their maturation and physiological status. We here characterized MG on three levels, including (a) global image-level, (b) apicobasal texture, and (c) regional apicobasal vertical-to-horizontal alignment. Using GliaMorph we quantified MG development on a global and single-cell level, showing increased feature elaboration and subcellular morphological rearrangement in the zebrafish retina. As proof-of-principle, we analysed expression changes in a mouse glaucoma model, identifying subcellular protein localization changes in MG. Together, GliaMorph enables an in-depth understanding of MG morphology in the developing and diseased retina.

Type: Article
Title: GliaMorph: A modular image analysis toolkit to quantify Müller glial cell morphology
Location: England
Open access status: An open access version is available from UCL Discovery
DOI: 10.1242/dev.201008
Publisher version: https://doi.org/10.1242/dev.201008
Language: English
Additional information: © 2023. Published by The Company of Biologists Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed
Keywords: Development, Glia morphology, Müller glia, Retina, Zebrafish
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > Institute of Ophthalmology
URI: https://discovery.ucl.ac.uk/id/eprint/10163290
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