UCL Discovery
UCL home » Library Services » Electronic resources » UCL Discovery

Diagnostic accuracy of 1p/19q codeletion tests in oligodendroglioma: a comprehensive meta-analysis based on a Cochrane Systematic Review

Brandner, S; McAleenan, A; Jones, HE; Kernohan, A; Robinson, T; Schmidt, L; Dawson, S; ... Kurian, KM; + view all (2021) Diagnostic accuracy of 1p/19q codeletion tests in oligodendroglioma: a comprehensive meta-analysis based on a Cochrane Systematic Review. Neuropathology and Applied Neurobiology 10.1111/nan.12790. (In press).

[thumbnail of Neuropathology Appl Neurobio - 2022 - Brandner - Diagnostic accuracy of 1p 19q codeletion tests in oligodendroglioma  a.pdf] Text
Neuropathology Appl Neurobio - 2022 - Brandner - Diagnostic accuracy of 1p 19q codeletion tests in oligodendroglioma a.pdf - Accepted Version
Access restricted to UCL open access staff until 27 December 2022.

Download (1MB)

Abstract

Codeletion of chromosomal arms 1p and 19q, in conjunction with a mutation in the isocitrate dehydrogenase 1 or 2 gene, is the molecular diagnostic criterion for oligodendroglioma, IDH-mutant and 1p/19q-codeleted. 1p/19q codeletion is a diagnostic marker and allows prognostication and prediction of the best drug response within IDH-mutant tumours. We performed a Cochrane review and simple economic analysis to establish the most sensitive, specific, and cost-effective techniques for determining 1p/19q codeletion status. Fluorescent in situ hybridisation (FISH) and PCR-based loss of heterozygosity (LOH) test methods were considered as reference standard. Most techniques (FISH, CISH, PCR, Real-time PCR, MLPA, SNP array, CGH, array CGH, next generation sequencing, mass spectrometry, and NanoString) showed good sensitivity (few false negatives) for detection of 1p/19q codeletions in glioma, irrespective of whether FISH or PCR-based LOH was used as the reference standard. Both NGS and SNP array had a high specificity (fewer false positives) for 1p/19q codeletion when considered against FISH as the reference standard. Our findings suggest that G-banding is not a suitable test for 1p/19q analysis. Within these limits considering cost per diagnosis, and using FISH as a reference, multiplex ligation probe amplification (MLPA) was marginally more cost-effective than other tests, although these economic analyses were limited by the range of available parameters, time horizon and data from multiple health care organisations.

Type: Article
Title: Diagnostic accuracy of 1p/19q codeletion tests in oligodendroglioma: a comprehensive meta-analysis based on a Cochrane Systematic Review
Location: England
DOI: 10.1111/nan.12790
Publisher version: https://doi.org/10.1111/nan.12790
Language: English
Additional information: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.
Keywords: 1p/19q codeletion, Fluorescent in situ hybridisation, Oligodendroglioma, PCR, false positive, false-negative
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > UCL Queen Square Institute of Neurology
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > UCL Queen Square Institute of Neurology > Neurodegenerative Diseases
URI: https://discovery.ucl.ac.uk/id/eprint/10141704
Downloads since deposit
1Download
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item