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Intrinsic brainstem circuits comprised of Chx10-expressing neurons contribute to reticulospinal output in mice

Chopek, JW; Zhang, Y; Brownstone, RM; (2021) Intrinsic brainstem circuits comprised of Chx10-expressing neurons contribute to reticulospinal output in mice. Journal of Neurophysiology , 126 (6) pp. 1978-1990. 10.1152/jn.00322.2021. Green open access

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Abstract

Glutamatergic reticulospinal neurons in the gigantocellular reticular nucleus (GRN) of the medullary reticular formation can function as command neurons, transmitting motor commands to spinal cord circuits to instruct movement. Recent advances in our understanding of this neuron-dense region have been facilitated by the discovery of expression of the transcriptional regulator, Chx10, in excitatory reticulospinal neurons. Here, we address the capacity of local circuitry in the GRN to contribute to reticulospinal output. We define two sub-populations of Chx10-expressing neurons in this region, based on distinct electrophysiological properties and somata size (small and large), and show that these populations correspond to local interneurons and reticulospinal neurons, respectively. Using focal release of caged glutamate combined with patch clamp recordings, we demonstrated that Chx10 neurons form microcircuits in which the Chx10 local interneurons project to and facilitate the firing of Chx10 reticulospinal neurons. We discuss the implications of these microcircuits in terms of movement selection.

Type: Article
Title: Intrinsic brainstem circuits comprised of Chx10-expressing neurons contribute to reticulospinal output in mice
Location: United States
Open access status: An open access version is available from UCL Discovery
DOI: 10.1152/jn.00322.2021
Publisher version: https://doi.org/10.1152/jn.00322.2021
Language: English
Additional information: Copyright © 2021 The Authors Licensed under Creative Commons Attribution CC-BY 4.0. Published by the American Physiological Society.
Keywords: Interneurons, locomotion, microcircuits, reticular formation, spinal cord
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > UCL Queen Square Institute of Neurology
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > UCL Queen Square Institute of Neurology > Department of Neuromuscular Diseases
URI: https://discovery.ucl.ac.uk/id/eprint/10139572
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