Richards, DA;
Thomas, MR;
Szijj, PA;
Foote, J;
Chen, Y;
Nogueira, JCF;
Chudasama, V;
(2021)
Employing defined bioconjugates to generate chemically functionalised gold nanoparticles for in vitro diagnostic applications.
Nanoscale
, 13
pp. 11921-11931.
10.1039/d1nr02584h.
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Abstract
Novel methods for introducing chemical and biological functionality to the surface of gold nanoparticles serve to increase the utility of this class of nanomaterials across a range of applications. To date, methods for functionalising gold surfaces have relied upon uncontrollable non-specific adsorption, bespoke chemical linkers, or non-generalisable protein–protein interactions. Herein we report a versatile method for introducing functionality to gold nanoparticles by exploiting the strong interaction between chemically functionalised bovine serum albumin (f-BSA) and citrate-capped gold nanoparticles (AuNPs). We establish the generalisability of the method by introducing a variety of functionalities to gold nanoparticles using cheap, commercially available chemical linkers. The utility of this approach is further demonstrated through the conjugation of the monoclonal antibody Ontruzant to f-BSA–AuNPs using inverse electron-demand Diels–Alder (iEDDA) click chemistry, a hitherto unexplored chemistry for AuNP–IgG conjugation. Finally, we show that the AuNP–Ontruzant particles generated via f-BSA–AuNPs have a greater affinity for their target in a lateral flow format when compared to conventional physisorption, highlighting the potential of this technology for producing sensitive diagnostic tests.
| Type: | Article |
|---|---|
| Title: | Employing defined bioconjugates to generate chemically functionalised gold nanoparticles for in vitro diagnostic applications |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1039/d1nr02584h |
| Publisher version: | http://dx.doi.org/10.1039/d1nr02584h |
| Language: | English |
| Additional information: | This article is licensed under a Creative Commons Attribution 3.0 Unported Licence |
| Keywords: | BINDING, PLASMA, CONJUGATION, CHEMISTRY, PROTEIN, BLOOD, NANOMATERIALS, DELIVERY, ALBUMIN, BSA |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > UCL BEAMS UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences > Dept of Chemistry UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences > London Centre for Nanotechnology |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10130853 |
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