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Gas Phase Stability and Unfolding of Proteins via Ion Mobility-Mass Spectrometry

Eldrid, Charles; (2021) Gas Phase Stability and Unfolding of Proteins via Ion Mobility-Mass Spectrometry. Doctoral thesis (Ph.D), UCL (University College London). Green open access

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Abstract

The study of protein dynamics and unfolding is key to understanding the basis of misfolding and aggregational diseases such as type II diabetes, α1-antrypsin deficiency, Parkinsons’ disease and Alzheimers' disease. Common structural biology techniques, such as x-ray crystallography and cryo electron microscopy often require large quantities of high purity sample and can only capture major structural ensembles. Mass spectrometry coupled to ion mobility (IM-MS) is positioned as an ideal technique to study protein dynamics and unfolding as it allows the separation of ions based on mass, charge and conformation from low concentrations of heterogenous solutions. In this thesis I present new techniques, instrumentation and computational workflows relating to IM-MS and how these techniques can be applied to protein systems involved in misfolding and aggregational diseases.

Type: Thesis (Doctoral)
Qualification: Ph.D
Title: Gas Phase Stability and Unfolding of Proteins via Ion Mobility-Mass Spectrometry
Event: UCL (University College London)
Open access status: An open access version is available from UCL Discovery
Language: English
Additional information: Copyright © The Author 2021. Original content in this thesis is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0) Licence (https://creativecommons.org/licenses/by-nc/4.0/). Any third-party copyright material present remains the property of its respective owner(s) and is licensed under its existing terms. Access may initially be restricted at the author’s request.
UCL classification: UCL
UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
URI: https://discovery.ucl.ac.uk/id/eprint/10126866
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