Bhartiya, A;
Batey, D;
Cipiccia, S;
Shi, X;
Rau, C;
Botchway, S;
Yusuf, M;
(2021)
X-ray Ptychography Imaging of Human Chromosomes After Low-dose Irradiation.
Chromosome Research
, 29
pp. 107-126.
10.1007/s10577-021-09660-7.
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Abstract
Studies of the structural and functional role of chromosomes in cytogenetics have spanned more than 10 decades. In this work, we take advantage of the coherent X-rays available at the latest synchrotron sources to extract the individual masses of all 46 chromosomes of metaphase human B and T cells using hard X-ray ptychography. We have produced ‘X-ray karyotypes’ of both heavy metal–stained and unstained spreads to determine the gain or loss of genetic material upon low-level X-ray irradiation doses due to radiation damage. The experiments were performed at the I-13 beamline, Diamond Light Source, Didcot, UK, using the phase-sensitive X-ray ptychography method.
| Type: | Article |
|---|---|
| Title: | X-ray Ptychography Imaging of Human Chromosomes After Low-dose Irradiation |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1007/s10577-021-09660-7 |
| Publisher version: | https://doi.org/10.1007/s10577-021-09660-7 |
| Language: | English |
| Additional information: | Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. - Please note: This is a corrected version of the article published on 06 August 2021. |
| Keywords: | X-ray imaging, X-ray microscopy, Karyotype, Irradiation, Chromosome structure, Mass determination |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > UCL BEAMS UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences > Dept of Chemistry UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences > London Centre for Nanotechnology |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10126018 |
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