Märk, J;
Dortay, H;
Wagener, A;
Zhang, E;
Buchmann, J;
Grötzinger, C;
Friedrich, T;
(2018)
Dual-wavelength 3D photoacoustic imaging of mammalian cells using a photoswitchable phytochrome reporter protein.
Communications Physics
, 1
, Article 3. 10.1038/s42005-017-0003-2.
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Abstract
Photoacoustic imaging has been shown to provide high-resolution images of genetically labelled cells at depths that are inaccessible to optical microscopy. While the detection of genetic reporters, such as fluorescent proteins and pigments, has been demonstrated using multiwavelength imaging and spectral unmixing, these approaches remain challenging due to their large computational scale. In this study we report a method based on a reversibly photoswitchable phytochrome-based reporter protein (AGP1) and dual-wavelength interleaved image acquisition for obtaining difference images with unambiguous reporter-specific contrast. Detailed, full 3D images of tumours formed of cells lentivirally transduced to express AGP1 were acquired in vivo in deep tissue in a longitudinal study. This method represents a powerful new approach to studying cellular and genetic processes which, due to its experimental simplicity, can be implemented in a wide range of existing photoacoustic imaging platforms.
| Type: | Article |
|---|---|
| Title: | Dual-wavelength 3D photoacoustic imaging of mammalian cells using a photoswitchable phytochrome reporter protein |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1038/s42005-017-0003-2 |
| Publisher version: | https://doi.org/10.1038/s42005-017-0003-2 |
| Language: | English |
| Additional information: | This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
| Keywords: | Biophotonics, Photoacoustics |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > UCL BEAMS UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Med Phys and Biomedical Eng |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10123460 |
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