Gu, Mei; (1998) Mitochondrial function in Parkinson's disease and other neurodegenerative diseases. Doctoral thesis (Ph.D), UCL (University College London).

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Abstract

The cause of neuronal loss in the substantia nigra from Parkinson's disease (PD) brain is unknown. A deficiency of mitochondrial respiratory chain (MRC) complex I has been found in PD substantia nigra. Normal complex I function in multiple system atrophy suggested neither neuronal degeneration nor L-dopa therapy was the cause of this defect in PD. Normal MRC function in other specific brain areas from Alzheimer's disease (AD), dementia with Lewy bodies (DLB) and PD suggested that neither cholinergic cell loss nor the presence of Lewy bodies perse was associated with complex I deficiency. The increased ApoE ϵ4 allele frequency in AD and DLB was not observed in PD patients with dementia. These results suggested that the ApoE ϵ4 allele influenced neither the development of Lewy bodies nor the dementia associated with PD, and that the risk factors for dementia in PD differed from that of AD and DLB at least with respect to ApoE. In Huntington's disease (HD) caudate nucleus, severe defects of complexes II, III and complex IV activities of the MRC were demonstrated, supporting the role of abnormal energy metabolism in HD. The feasibility of the platelet-A549ρ° cell fusion technique to study the involvement of mitochondrial DNA (mtDNA) in PD was demonstrated using platelets with the A3243G mtDNA mutation (MELAS). Platelets from seven PD patients with low complex I activity were fused with A549ρ° cells. Mixed cybrid analysis demonstrated a selective 25% deficiency of complex I activity. Furthermore, the analysis of 16 A549ρ°-PD fusion cybrid clones from one of the patients expressed complexes I (25%) and IV (20%) deficiencies. These results point to abnormal mtDNA as the underlying cause of the MRC dysfunction in at least a proportion of PD patients. The MRC inhibitors piericidin A and antimycin A induced much greater levels of apoptosis in A549 than in A549ρ° cells implying apoptosis was induced via a mechanism that involved inhibition of the MRC, this contrasted with the toxic effects of rotenone which affected both cell types equally and therefore must be mediated via a pathway independent of the MRC.

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